X, B, 1 Ruediger: Preparation of Tetanus Antitoxin 45 

Fic. 38. Chart showing the antitoxin curve for horse 4. Units per cubic centimeter by months. 
The injections of toxin were continued on horse 5. Jn the 
middle of May, 1914, the serum was tested and was found to 
contain only about 125 units of antitoxin per cubic centimeter. 
On May 19, 1914, the horse was bled 6 liters and after that 
was used for other purposes. 
The antitoxin content of the serum of horse 5 reached the 
highest recorded mark in the seventh month, fell about 50 units 
per cubic centimeter in the eighth month, and rose again to, or 
a little over, the previous height in the tenth month, and after 
that it began to fall. 
Horse 5 received a total of 18,433.5 cubic centimeters of teta- 
nus toxin and was bled 48 liters. During one year this horse 
produced about 19.2 liters of antitetanic serum or 4,430,000 units 
of tetanus antitoxin. After deducting 50 per cent for loss, we 
have left 2,215,000 units of antitoxin (see figs. 39 to 51, charts 
5—A to 5—M, for horse 5). 
Tetanus No.5 A 
[zel27[2s]2y [so] | 
ra 1A = aan 
TAY A 
ae Pk, Sreiate 
a WAT 
fH yey 
aL 
Cy 
Fic. 39. Temperature chart of horse 5 for June, 1913. 













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