X, B, 1 Ruediger: Preparation of Tetanus Antitoxin 53 
cubic centimeter; the doses were gradually increased and given 
at intervals of a week. In three months the dose was increased 
to 100 cubic centimeters. On March 9, 1914, the serum con- 
tained 250 units of tetanus antitoxin per cubic centimeter. In 
April, 1914, the horse was bled 11 liters—6 liters on April 6 
and 5 liters on April 10. The serum tested 500 units of anti- 
toxin per cubic centimeter. The injections of toxin were con- 
tinued. Five liters of blood were withdrawn on June 2, 1914, 
the serum of which contained 700 units of antitoxin per cubic 
centimeter. In July, 1914, the horse was bled four times, 5, 
12, 10, and 10 liters being obtained; the serum obtained tested 
700, 700, 600, and 500 units, respectively, per cubic centimeter. 
Three injections of tetanus toxin were given, then 19 liters 
of blood were withdrawn: 10 liters on August 11, 1914, and 
9 liters on August 14, 1914. The serum contained 500 units 
and 400 units, respectively. Nine liters of blood withdrawn 
on September 1, 1914, yielded 3.5 liters of serum which con- 
tained 250 units of antitoxin per cubic centimeter. Nine liters 
of blood obtained on September 4, 1914, produced 4.4 liters 
of serum with 225 units of tetanus antitoxin per cubic centi- 
meter. A third bleeding of 5 liters on September 7, 1914, yielded 
2.5 liters of serum with about 200 units of antitoxin per cubic 
centimeter. On September 9, 1914, the horse was bled to death. 
Nineteen liters of blood were obtained, which yielded 10 liters 
of serum that contained 150 units of tetanus antitoxin per cubic 
centimeter. 
The antitoxin content of the serum of horse 7 reached its 
highest recorded mark in seven months. This horse received a 
total of 14,619.5 cubic centimeters of tetanus toxin and furnished 
114 liters of blood. It produced 51.7 liters of antitetanic serum, 
or 21,315,000 units of tetanus antitoxin. If we consider 50 
per cent of the antitoxin as lost through deterioration and other 
accidental causes, we can still count on marketing 10,657,500 
units of tetanus antitoxin produced by 1 horse in ten months (see 
figs. 62 to 73, charts 7—A to 7—-L, for horse 7). 
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Fic. 62. Temperature chart of horse 7 for November, 1913. 
