x, B,1 Ruediger: Preparation of Tetanus Antitoxin 61 
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Fic. 85. Chart showing the antitoxin curve for horse 8. Units per cubie centimeter by months. 




CONCLUSIONS 
From the results obtained and reported above, the following 
conclusions seem justified: 
1. A suitable strain of the bacillus of tetanus will usually 
produce potent toxin when grown in nearly neutral glucose broth 
under hydrogen. The acidity of the broth will rise to more 
than 2 per cent normal acid, and it should be neutralized with 
sodium hydrate before it is injected into the horse. 
2. Potent tetanus toxin was obtained by the method described 
by Ivan Hall. By this method the acid is continuously neutral- 
ized by the magnesium carbonate present. 
3. Horses differ greatly in the power of producing tetanus 
antitoxin. Of 8 horses reported on, one produced 150 units per 
cubic centimeter of serum, two produced 300 units per cubic 
centimeter of serum, one produced 350 units per cubic centi- 
meter, and in the serum of one the antitoxin rose to 400 units 
per cubic centimeter. Three horses produced 500 or more units 
of tetanus antitoxin per cubic centimeter of serum. 
4. The antitoxin curve reached its highest mark in from six 
to nine months after the beginning of immunization. 
5. The injection of large doses of toxin is not indicated. The 
dosage should be such that the horse does not appreciably lose 
in weight. 
