X, B, 2 Schébl: Diagnosis of Asiatic Cholera 129 
TABLE I.—Hnrichment cultures incubated eighteen hours. 








_ Enrichment. Growth 
pupae: Remarks. 
Medium. Reaction.| agar. 
du =— | 
+1.5 = on acid producers present besides cholera. 
+1 == 
+0.5 + 
—0.5 aR Their number decreases toward the alkaline end of 
= a the series of peptone. Platesmadefrom peptone | 
S15) + +2, +1.5, and +1 overgrown by acid producers. 
—2 + 
| 


TABLE II.—Same arrangement of experiment as in Table I. 






i} 
Enrichment. Growth | 
jon plates. | 
} Dieu- Remarks. | 
Medium. bea cone 5 
IReptoness eee ae a + 
)OX0) ee es es eee +1.5 a | 
I DY yee ae es tome eae 54] — Overgrown by a motile bacillus growing fairly well 
DOr eee eee, eck | +0.5 — on Dieudonné’s plates. One single colony found 
Tare She ea ee | (us — |{ on the plate made from +2 peptone. It proved 
1D) eats ee | = = || to be one of cholera vibrio. 
1D) See ee tab Soa ja 
1D) eee ee eee | —2 | = | 
i 

TABLE III.—Same arrangement as Table II. Both lactose agar and Dieu- 
donné’s plates used. Incubation of peptone culture, eighteen hours. 







Enrichment. Growth on plates. 
Ey = Dieta Remarks. 
: . actose | Dieudon- 
Medium. Reaction. sre, aOR aE. 
=r Few acid producers from +2. The num- 
4.5 ber of red colonies decreases toward the 
aril alkaline end of series. No blue colonies 
+0.5 except cholera. 
=1 Cholera colonies most numerous on plates 
=e made from peptone of from —0. 5 to —1.5 
= reaction. 
Sed | Sear | + ae one cholera colony +2 plate. Plates 
6 ER meee =F show pure growth of cholera. 
tal oP 
+0.5 t 
0.5 iE Cholera colonies most numerous on plates 
so made from peptone of from —0. 5to— 1.5 
Tis [LT] E |p eect 
a) | eae + 





