142 The Philippine Journal of Science 1915 
TABLE XVI.—Same arrangement as Table XI. Eighteen hours’ incuba- 
tion—Continued. 







| i 
Enrichment. ere | | 
| 
| a eae | | K | =, - Remarks. 
Medium. | Tec centr Ts ldonné’s| 
: tion. | agar. | agar. | 
nue ieee 
| | 
Per ct. 
Kraus’ spo owes |t Sea | 10 jo AR [eesessce More than a few cholera colonies; equal 
number of red ones. 
DY Reeias ar ie See eS Sos eS Dal een een | Few cholera colonies; equal number 
| of red ones. 
Soda peptone ________ 1 eee: ae Sf | ee ee More than a few cholera colonies; few 
| white ones. 
Do Seas wee ee <2 eee Sear | a tall See | Few small white colonies; no cholera. 
Domes Se ees) ate |e gs eg eet tea Do. 
Peptone!sene eee | ey (ae ee oe np eae ee = | No growth. 
Do estes a oes = Oi 52a eee [Sie ame + | More than a few cholera colonies, pure. 
Do = ees } —1 | Sa ae i oe + | Do. 
Doss see ee | —2 eeoeae 1 ak + | Numerous cholera colonies, pure. 
Ottolenwhitss: 220 ood ae ee a eee ee = No growth. 
SEN USES aera eet eae | es te GSH ee ee ee + Very numerous cholera colonies, pure. 
Dosa arenes poate Sn ALO) eee + Do. 
Dye se aoe ge {So eee GS | eee + More than a few cholera colonies, pure. | 
Soda peptone ________ | —— 1 oe eee Se Ree + Numerous cholera, pure. | 
Do ee eee Wet ety eet pear 2! = No growth. 
| Dot een a ri Pita ae ee = Do. 






SUMMARY 
It will be seen from the protocols that the bacteria other 
than cholera vibrios, as encountered in cholera stools, can be 
divided into three groups from the practical standpoint, namely: 
1. Bacteria which prefer an acid medium to an alkaline one; 
they are lactose fermenters—that is, representatives of the 
coli group. They were most frequently met with. Fortunately 
this class of bacteria can be successfully eliminated, or at least 
reduced so as not to interfere with the growth of the cholera 
vibrio, by alkaline reaction of the enrichment medium. They 
do not grow on Dieudonné’s medium. 
2. Bacteria which prefer strong alkaline reaction. They do 
not acidify lactose and are of little importance. Being cocci, 
they form small colonies, grow slowly, and occur in small 
numbers on the plates. They grow on Dieudonné’s medium 
(Tables: 1V,; Vi,. Vil, X,. XI, XI, Na RA, AV Cie 
3. Bacteria which exhibit the same tolerance toward alkaline 
reaction of the medium as the cholera vibrio does and which 
have a broad range of growth in regard to the reaction of the 
medium. They were found to form numerous, some of them 
