® 
X, B, 2 Barber: Experiments on Immunization 147 
The results of all series are fairly uniform, and the discussion 
of this, as of Tables II, III, and. IV, will be found after Table IV. 
TABLE I.—Series 1. Comparatively small avirulent dose, part avian, part 


























human. 
Weicht, |Aug. 6, 1912;16days) Sept. 23, 1912; 110 
Virulent dose, Dec. 20, 1911. June 5, after virulent days after virulent 
, 1912. In- dose. dose. 
Guinea ee ee = oculated 
pig No. anil 
Strain. Wels Weebl Manes | mena.) Weight. | PBURAll Weighe. 
cc. g g. 9 0 
6080) |) “Lbhas Avan _- = - 3 400 460 St BOOUN S- 2 das eS Sle Somes oe 
(ROB ee dope aes 3 460 610 ae 640 APP 640 
OBR Hesceel GOP ea esate ne 3 6400) Sse S| ee See Se a ae le Jee 
GOSSue|aaeee (600) ee eae Zap 490 500 ar 480 ar 410 
6034 | Emuls avian only__.. 1 390 560 + 610 BF 670 | 
60385 | Thag He ____.._.____ 3 450 | 650 + 620) | eeao | 
| 6086 |____. dopeeen ee ua 3 660 630 + 650| ++ | 660 | 
OE eet (so) Se rere Sm 3 420 410 = 480 srapar || 520 | 
GOSBR i se (6 (0), DE eee ee 8 || 500 | A10 ts 450 Ir | 450 
6089 |--do ie Bes) De ee) 0) | Seo | 
Average_._____|___.___- [aso Aa a iy |e ee | 
Nov. 16, 1912; 164 | Dec. 19, 1912; 197 Autopsy. 
days after virulent] days after virulent 
dose. dose. Died ti 
Guinea =e after |. Ratio of 
piste I inal A | ee Boy Spleen. Tesiona. rents 
neuinal) Weight. | meuinal) weight. ee roa, 
g- - g. Days. g. g. 
SURO | pceneceee RRS Pe rete freee eae ae Lees es a 88 360 0.6 Tb. 0. 0016 
6031 aa beh Sas eee Se ee 179 510 4,2 Th. 0. 0082 
DUBVA 9 fers eee ese eer a a pee py ee Be eee see oe (Db) Ae ene 
CUI) Ns 2 ee ee el eee ee 133 320 2.5) Tb. 0. 0078 | 
6034 + 680 SF 640 349 480 B15) Tb. 0. 0073 
6035 SSF 690 Sie 740 377 580 1.6 Tb. 0. 0027 
6036 ar ar 650 sae 640 311 470 2.6 4loy 0. 0055 
6037 simaieaia 470 a aR ae 420 | 222 370 3.5 Tb. 0. 0095 
6038 ar 410) | oct le ese 187 340 5.4 Tb. 0. 0159 
6039 =F 740 sear 730 283, 620 2.0 Ao 0. 0032 
Ah Seas Ee oS TS Go REG |sonceccecnce eee aca 
— 
8 Tbag A and Tbhag H refer to mixtures of agar with avian and human types of bacilli, 
respectively. ; , 
> Sacrificed. 
Series 2, Table I1J.—In this series one hundred twenty-seven 
days intervened between the immunizing and the avirulent doses. 
For immunization only the human avirulent strain was used, and 
the animals were divided into four groups. The first group, 
“Tbhag a,” received an agar mixture prepared as follows: Three 
cubic centimeters of a thick emulsion from a 40-day culture was 
132716——4 
