X, B,2 Barber: Experiments on Immunization 161 
SERIES D 
Five guinea pigs were inoculated August 16, 1913, with spleen 
pulp of a case of human leprosy. Acid-fast bacilli were not 
found in the human spleen. One animal died of sepsis soon 
after inoculation. None of the other four developed any signs 
of tuberculosis. 
SERIES E 
Five guinea pigs were inoculated August 18, 1913, with spleen 
pulp of a case of leprosy, tubercular form. Numerous bacilli 
lepree were found in the spleen. Of these, three animals show 
no signs of tuberculosis after over one year’s observation. One 
died about one year after inoculation with no signs of tuber- 
culosis, and one died forty-two days after inoculation, with en- 
larged inguinal glands, and apparently tubercles in the lungs 
and spleen, but acid-fact bacilli were not found in smears from 
the inguinal glands. Probably this animal died of some other - 
disease. 
SUMMARY ° 
1. Five series of guinea pigs or monkeys were inoculated with 
the spleen pulp of lepers taken at post mortem. Lesions of 
tuberculosis or lesions very similar to those of tuberculosis de- 
veloped in two of these series. In one of the two series only 
part of the pigs developed tuberculosis. Inone (series E) 
one guinea pig out of five showed lesions, possibly those of 
tuberculosis. 
2. A series of guinea pigs inoculated with a strain of tuber- 
culosis of leper-spleen origin (series A) exhibited at post mortem 
a remarkable enlargement of the spleen. The average ratios of 
the spleen weight to the body weight at post mortem were nearly 
double the average ratios of a series dying of tuberculosis of 
human-sputum origin. 
