2 The Philippine Journal of Science 1915 
in Algeria it appears from reports received from the American 
Consul at Algiers (see page 175) that no satisfactory field 
results were obtained even from a virus that had passed through 
from 74 to 8&7 grasshoppers. In laboratory experiments, how- 
ever, Many passages apparently were necessary, in the Algerian 
grasshoppers, to exalt the virus to a sufficient degree. According 
to the report mentioned above, the virus, at first not certainly 
fatal after a period of from twenty-four to thirty-six hours 
following artificial inoculation, was exalted to a degree where it 
was invariably fatal within four hours. 
Experiments on locusts with Coccobacillus acridiorum were 
continued in May, 1914, in Mindoro by one of us (Barber). A 
new culture obtained from Argentina was used and submitted to 
23 locust passages. In the laboratory experiments in this series 
there was more tendency among infected insects to discharge 
liquid excrement than was observed in the experiments of 1913 
in Luzon, but this diarrhoea was by no means a constant symptom. 
Field experiments were conducted with cultures from insects 
where the bacterial dose (diluted ten times) was sufficiently 
strong to kill inoculated insects within six hours. 
In the field experiments infection was attempted on both 
winged locusts and “hoppers.” The cultures were applied to the 
grass or cane on which the insects were feeding in several dif- 
ferent ways: namely, spraying or broth cultures alone, broth 
cultures plus meal and molasses, and the extract from dead 
crushed insects. These were taken from a large cage, where 
they had been fed on a presumably exalted virus. Locusts 
confined in cages during this series of experiments fed much 
better than during our experiments of 1913. Grass soaked in 
culture media was devoured immediately, and healthy insects 
readily fed on the dead ones. So, in order to get a further 
method of spreading the infection in the field, locusts were 
caught, fed on culture-soaked grass, and turned loose among 
the field swarms. 
In not a single instance during the whole of the Mindoro 
experiments was there the slightest evidence of the spread of 
infection among insects in the field. During the experiments the 
weather was hot with frequent afternoon showers. 
On the application of the Bureau of Science for information 
regarding the practical success with Coccobacillus acridiorum 
obtained in other countries, the following reports from consuls 
in Argentina, Columbia, and Algeria were transmitted through 
the Government at Washington to the Bureau of Science. These 
are given verbatim. 
