x,B,2 Barberand Jones: Coccobacillus acridiorum d@Herelle 175 
Consul at Algiers, Algeria, giving the result of experiments that have 
been made in Algeria with Coccobacillus acridorium of d’Herelle.. 
Reference is made to a letter on this subject, dated April 18, 1914, 
from the Assistant Secretary of War. 
Inclosure: From Algiers, May 2, 1914, with inclosure. 
LOSE 92: 
[First indorsement.] 
BUREAU OF INSULAR AFFAIRS, 
May 28, 1914. 
To the Governor-General of the Philippine Islands, Manila, P. I. 
co. 
Incl. 6478-41. 
[Second indorsement.] 
THE GOVERNMENT OF THE PHILIPPINE ISLANDS, 
EXECUTIVE BUREAU. 
Le 
MANILA, June 29, 1914. 
Through the Director of the Bureau of Science, to the Director of Agri- 
culture. 
EXPERIMENTS IN ALGERIA ON GRASSHOPPERS WITH THE COCCOBACILLUS 
ACRIDORIUM OF D’HERELLE 
In 1913 Professor Sergent of the Pasteur Institute of Algeria com- 
menced investigations to ascertain whether the destruction of the grass- 
hopper indigenous in Algeria, the Stauronotus maroccanus Thunberg, could 
be effected by the bacillus of d’Herelle. 
As the results obtained were considered inconclusive further experiments 
are being made at present the results of which will be duly reported when 
experiments are concluded. 
The following information was obtained through a personal interview 
with Professor Sergent. 
It was found that injections of cultures containing the bacillus d’Herelle 
sometimes caused the death of grasshoppers in from 24 to 36 hours but 
that in other instances injections were not fatal. It was further found 
that the virulence of bacillus was increased in the bodies of grasshoppers 
and that by injections of bacillus from one grasshopper to another the 
virulence of the bacillus was increased to such a degree that death could 
invariably be caused within four hours of infection. After transmission 
of virus through 28 insects the average life of insect after infection was 
seven hours, after transmission through 70 insects 6 hours, and after 
transmission through 100 insects four hours. 
The process of infection from one grasshopper to another had to be 
carried on 20 days to obtain a regular mortality in seven hours after in- 
fection, one month for six hours and seven weeks for four hours. 
Two tests were made by Professor Sergent in the Department of Oran 
in 1913 to determine the practical value of infection of grasshopper with 
the bacillus d’Herelle. 
From the 15th to the 22d of April an area of about 40 acres was sprayed 
with 37 liters of bouillon containing virulent bacilli of d’Herelle, in the 
line of march of a column of grasshoppers. From the first day that spray- 
ing was commenced dead grasshoppers were found infected with bacillus. 
On the 30th of April large numbers of dead grasshoppers were found 
but the great majority of insects constituting the column were not affected. 
