320 The Philippine Journal of Science 1915 
conditions, was not in a state of proliferation but that of 
dying out. 
The presence of other bacteria in the mash made it difficult 
for B. typhosus to exist in competition with them. 
Graham-Smith, (42) in 1911, made observations on the ways 
in which artificially infected blowflies (Sarcophagide) carry 
and distribute pathogenic and other bacteria. 
Larve of the blowfly were fed on meat artificially infected 
with the spores of anthrax bacilli. Flies emerging from these 
larvee were infected with anthrax bacilli and remained infective 
for fifteen days. 
Larvee were fed on meat infected with the respective nonspore- 
bearing organisms, B. typhosus, B. enteritidis, B. prodigiosus, 
Vibrio cholerz, and a pink-colonied coccus. Examination of the 
emerging flies did not reveal the presence of any of these 
organisms. 
This indicates that nonspore-bearing organisms do not sur- 
vive sufficiently long to be found in the emerging blowflies. 
Later, in 1911, Graham-Smith(48) made more extensive ob- 
servations along these lines and employed the larve of Calli- 
phora erythrocephala, Lucilia cesar, and Musca domestica. 
SERIES 1 
Graham-Smith, failing to infect the emerging flies from larve 
fed on artificially infected food, secured larve of C. erythroce- 
phala and L. cesar and fed them upon the flesh of guinea pigs 
which had died from infection with B. enteritidis and B. an- 
thracis. Several emerging flies when examined did not show the 
presence of spore-free anthrax bacilli or of B. enteritidis. 
SERIES 2 
Here the females of M. domestica were allowed to lay their 
eggs upon a mixture of boiled meat, potatoes, and rice. Indi- 
vidual groups of larve were secured and placed in separate 
sterile sand boxes, and each was fed respectively on B. prodi- 
giosus, Morgan’s bacillus, B. enteritidis, B. anthracis, and a pink- 
colonied coccus. The intestinal contents of the emerging flies 
when examined showed negative results in the following: B. 
prodigiosus, B. enteritidis, and the coccus. Examination of the 
flies emerging from larve fed on Morgan’s bacillus gave a 
positive result. Of those emerging from larve fed on anthrax 
bacilli, positive results occurred in 78 per cent of the cases. 
These results indicate that nonspore-bearing organisms, such 
as B. prodigiosus, B. enteritidis, and certain cocci, cannot sur- 
