324 The Philippine Journal of Science 1915 
were entirely black and smaller in size. These were regarded 
by Banks as males of Aphiochzta. Brunetti also states in his 
description of Aphiochxia ferruginea that there is a variance in 
color. 
When a medium of sand was employed, containing food in the 
form of sugar, bread, human feces, and bouillon, the eggs de- 
veloped into a great number of flies, the great majority of 
which were the brownish yellow Aphiocheta. There were, also, 
present fewer numbers of those having a blackish appearance. 
To identify the flies, a considerable number were secured im- 
mediately upon emergence and studied with a magnifying glass. 
Most of those examined were found to be female Aphio- 
chxeta; others, the small and darker males. As it was impos- 
sible to examine all the flies which emerged by this method, it 
was possible that other allied species were present whose simi- 
larity might cause them to be confused with Aphiocheta. 
The period of development could be definitely ascertained, 
because when the flies emerged they were secured and identified 
as Aphiocheta. 
In series II and III considerable difficulty was encountered 
where experiments were performed to ascertain whether larve 
when growing in a medium infected with cholera vibrios contain 
these organisms in their intestines, and whether a transference 
of vibrios occurs from the larve, through the pupe and into the 
emerging imagines. - 
Repeated attempts were made to keep larve alive on a medium 
containing a pure growth of cholera vibrios. Larvz could exist 
for only a day or two on growths of cholera vibrios on agar-agar 
and Dieudonné’s medium. On agar-slant growths of vibrios to 
which sand was added larve could subsist for four or five days. 
Larve could be kept alive for nine days on an agar-slant 
growth of cholera vibrios containing sterile sand liberally soaked 
with bouillon. In this case flies were allowed to enter the test 
tube containing the vibrio growth and sand by inserting a bent 
glass tube into the test tube and connecting it with a flask con- 
taining flies. The flies traveled from the flask into the tube, 
where they were imprisoned sufficiently long to allow them to lay 
their eggs, after which they were allowed to pass along a bent 
glass tube into another flask. The larve, although living for 
nine days, did not attain their full size nor did they pupate. 
From this tube 6-day-old larve were removed with a sterile 
platinum needle and examined to see whether their intestines 
contained cholera vibrios. To remove the surface vibrios, the 
