328 The Philippine Journal of Science 1915 
The bottle containing the agar-agar had but two flies. This 
was covered. 
The flies from these four traps were removed and placed in 
separate flasks for identification. For the most part these flies 
averaged 2.5 millimeters; some were smaller. Specimens of the 
larger type were compared with members of the Phoridz in 
the Bureau of Science collection, identified by Professor Banks. 
These flies corresponded with Aphiocheta ferruginea of that 
collection and coincided with Brunetti’s description. 
Before the present series of experiments was undertaken, ! 
had exposed, on March 4, a large gauze-covered specimen jar 
containing feces. In this jar hundreds of small flies had 
emerged. These proved to be Aphiocheta ferruginea, and were 
saved as a stock supply for future experiments. 
On April 1 great numbers of small larvz were seen crawling 
about the feeces-agar mixture in the bottle and beaker. Fewer 
were seen on the dead snail. On the plain agar they were not 
yet visible. 
On April 6, the bottle containing the feeces-agar mixture being 
small and having a great number of larve measuring from 2 to 
3 millimeters, the entire contents were placed into a large speci- 
men jar containing sand, lumps of sugar, bread, feces, and bouil- 
lon. Eight layers of cloth gauze (having 20 squares to the 
linear inch) were stretched over the orifice to prevent new flies 
from entering. 
Upon transferring the contents it was seen that a number of 
the larvz were pupating. Examination of larve which had 
not yet pupated showed on their blunt extremities, on the in- 
ferior portion thereof, two small hooks not unlike those described 
by Brunetti as appearing like two small walrus tusks. The body 
is whitish yellow, and the dark intestinal contents are visible 
through the body wall. 
On April 8 a newly emerged fly made its appearance. On the 
following day (April 9) flies appeared in great numbers. On 
examination the great majority were found to be Aphiochxta 
ferruginea. 
It is interesting to note that some of the flies escaped from 
the jar, passing through eight layers of gauze tightly stretched 
over the surface. 
The emerged flies were allowed to remain in the jar. On 
April 14 deposits of small eggs were seen on the sides of the jar. 
These are whitish with a tinge of yellow, and measure 0.2 by 0.5 
millimeter. The extremities are rather blunt, and there is a 
slight curve in the long axis. 
