106 



the solvent for the adrenaline was the saline solution employed 

 for comparison : — 



Table 4. 



Experiment No. 12. 



Solution. 



NaOl 



Adrenaline 1 : 

 NaCl 



Adrenaline 1 : 

 NaCl 



Adrenaline 1 : 

 Ergotinine 1 

 Adrenaline 1 : 

 NaCl 

 Ergotinine 1 



Solution. 



50NaCl+lCaCl 

 Adrenaline 1 : 10 

 50 NaCl+1 CaCl, 

 Adrenaline 1:10000 

 Adrenaline 1 : 1000 

 50NaCl+l CaCl, 





Post. 



Rect. 



Ant. Rect. 



Dist. 





Rect. 



Gl. 



and Valve. 



Int. 





... + 



+ 



+ 



+ 



1000 



... + 



+ 



+ 



+ + 





... + 



+ 



+ 



— 



1000 



... + 



' + 



+ 



+ + 



... 



... + 



+ 



+ 



_ 



1000 



... + 



+ 



+ 



+ + -+ 



1000 



... - 



- 



— 



_ 



1000 



... + 



+ 



+ 



+ 



... 



... + 



+ 



+ 



_ 



1000 



Table 



5. 



" 



" 





Experiment No. 42. 









Post. 



Rect. 



Ant. Rect. 



Dist. 





Rect. 



Gl. 



and Valve. 



Int. 



^Cl^ 



... + 



— 



- 



+ 



10000 



... — 



- 



+ 



+ + 



+ + 



The adrenaline employed in these experiments was the 

 free base prepared from suprarenal glands by the method of 

 Abel (15). The dosage required to- elicit these effects is high 

 compared with the dosages required to elicit responses in 

 mammalian tissue, nevertheless the effects are definite. The 

 optimum dosage varies in different preparations, just as 



the — optima vary. In some preparations 1 : 1000 adrenaline 



is requisite to elicit intestinal peristalsis ; in others this is an 

 excessive concentration, and leads to total immobilisation of 

 the intestine. If a preparation which has been thus immob- 

 ilised, however, be returned to the solution originally bathing 

 it and employed as solvent for the adrenaline, stimulation is 

 observed of such a pronounced character as to indicate an 

 after-effect, or possibly the effect of adrenaline imbibed by the 

 tissue and subsequently diluted by the adrenaline — free 

 solution. Adrenaline was, in fact, found to be a very useful 

 means of stimulating sluggish preparations to prolonged 

 activity. If the bathing solution was favourable to rectal 

 contractions, e.g., pure NaCl, this after-stimulation extended 



