﻿44 The Philippine Journal of Science i9i4 



is also under way to determine the practicability of substituting 

 cultures of rinderpest virus for virulent blood in simultaneous 

 inoculations. 



The results obtained present a wide range of possibilities for 

 the improvement of the present technique of immunization and 

 for the determination of the etiological factor, which may revise 

 the present methods of combating the disease. 



SUMMARY 



1. From the results obtained from various experiments it is 

 evident that the virus of rinderpest requires either partial or 

 complete anaerobic conditions for its existence. 



2. The virus of rinderpest has been carried in virulent form 

 in two separate series up to the sixth transfer in glucose-blood 

 culture media, covering periods of nineteen and twenty-one days, 

 respectively. 



3. In one series the medium first inoculated was nonvirulent 

 at the end of twelve days, while the fourth transfer from this 

 tube of culture medium after the same period of time was 

 virulent. 



4. Results obtained from numerous experiments indicate that 

 fresh blood from nonimmune cattle as a main constituent and 

 glucose as an addition are essential components of the culture 

 media. 



REFERENCES 



(1) Tartacovsky, M. M. Contribution a I'etiologie de la peste bovine. 



Arch. sci. bioL, St. Petersbourg (1896), 4, 295-327. 



(2) Nencki, M., Sieber, N., and Wijnikewitch, W. Recherches sur la 



peste bovine. Ibid. (1896), 4, 374-396. 



(3) Koch, R. Reise-Berichte iiber Rinderpest, etc. Julius Springer, Berlin 



(1898), 5-33, 56, 65; Report. Veterinary Journ. (1897), 45, 204; 

 Berichte des Herrn Prof. Dr. Koch iiber seine in Kimberley gemachten 

 Versuche beziiglich Bekamfung der Rinderpest. Centralbl. f. Bakt. 

 etc., 1 Abt. (1897), 21, 526. 



(4) Bass, C. C, and Johns, F. M. The cultivation of (Plasmodium vivax 



and Plasmodium falciparum) in vitro. Journ. Exp. Med. (1912), 

 16, 567. 



