﻿66 



The Philippine Journal of Science 



1914 



experiment are not in agreement. It has been observed ' that 

 blood drawn in the later stages of rinderpest did not transmit 

 the disease. 



INFECTIVITY OF GRASS AFTER SPRINKLING WITH URINE FROM 



INFECTED ANIMALS 



Experiment 16. — This experiment differed from the preceding 

 ones in that urine diluted with an equal amount of water was 

 sprinkled on grass and animals were allowed to graze after 

 various intervals. 



Five hundred cubic centimeters of urine were collected on 

 April 11, 1912, from bull 3361. At 4.30 p. m. on the twelfth 

 day after injection with virulent blood and the seventh day after 

 initial rise of temperature, the diluted mixture was sprinkled 



on the grass plot, which was left unoccupied for twenty-four 

 hours, the weather being dry and hot. 



On account of the expense involved, no control was used to 

 demonstrate the infectiveness of the urine at the time that it 

 was sprinkled on the grass. 



On April 12 at the same hour, bull 3365 was picketed over 

 the infected spot and left there twelve hours to graze, after 

 which it was placed in a stall. No rise of temperature was 

 observed after exposure; the susceptibility of the animal was 

 proved subsequently. 



In view of the absence of a control, the conclusion must be 

 of a conditional character. It can only be stated that urine, 

 voided at the stage of the disease indicated and left on gi-ass for 



'Ward and Wood, Bull P. I. Bur. Agr. (1912), No. 19, 59. 



