﻿IX, B, 1 Ward et al.: Transmission of Rinderpest 67 



twenty-four hours, did not convey infection to a susceptible 

 animal. 



Experiment 17. — This experiment continued the investigation 

 of the results following the sprinkling of grass with the urine 

 from an infected animal. A grass plot was used similar 

 to that in experiment 16, and three susceptible cattle were 

 employed. 



Urine was collected from two animals. Bull 3361, of the 

 previous experiment, furnished part, collected on the thirteenth 

 day after inoculation and eighth day of febrile temperature; 

 and part came from bull 3199 on the eighth day after inoculation 

 and fourth day after initial rise of temperature. 



The urine of both, amounting to 1,000 cubic centimeters, was 

 mixed and diluted to 2,000 cubic centimeters in water. The 

 grass plot was sprinkled with this mixture on April 12, 1912, 

 at 5 p. m., and was left unoccupied for thirty-six hours. The 

 weather on April 13 was cool, with showers in the forenoon, 

 and was clear and hot in the afternoon. 



On April 14, at 5 p. m., bull 3366 was picketed over the grass 

 plot and left there to graze until 2.30 p. m., after which it was 

 placed in a screened stall. 



During the forenoon the weather was cool, with showers. 

 Bull 3366 showed a rise of temperature on April 19, with 

 diarrhoea on April 23 and inappetence on April 25, followed 

 by recovery. 



It is concluded that rinderpest virus remained virulent on the 

 grass for at least thirty-six hours. 



Experiment 18. — This experiment was similar to the two 

 preceding ones. 



On the sixth day after inoculation, corresponding to the third 

 day of febrile temperature, 500 cubic centimeters of urine were 

 collected from bull 3363 and diluted to 1,000 cubic centimeters 

 with water. At 4.30 p. m., on April 16, 1912, this mixture was 

 sprinkled over grass and the plot was left unoccupied for forty- 

 eight hours. The weather during this interval was dry, hot, 

 and windy. No control was employed to test the infectiveness 

 of the urine at the time that it was sprinkled on the grass. 



On April 18, at 4.30 p. m., bull 3372 was picketed on the 

 grass and left there fourteen hours to graze, after which it 

 was placed in a screened stall. No rise of temperature occurred, 

 but the susceptibility of the animal was demonstrated later. 



The urine of bull 3363 was tested with negative results on 

 the seventh and eleventh days after inoculation in exactly the 



