﻿68 The Philippine Journal of Science i9i4 



same manner, except that the urine was exposed on grass only 

 twenty-four hours. The temperature of 3363 on each of these 

 dates was above 40° C. During both of these twenty-four-hour 

 periods the weather was dry, hot, and windy, with cloudless 

 sky. Both of the animals exposed to the grass plots failed to 

 contract rinderpest, but their susceptibility was proved later. 



It is concluded that if the urine of bull 3363 was virulent 

 when drawn, it was incapable of infecting a susceptible animal 

 after exposure on grass for forty-eight hours in one case and 

 for twenty-four hours in two instances. 



Experiment 19. — The purpose of this was similar to that 

 of those experiments immediately preceding. The urine was 

 voided by bulls 3368 and 3371. 



Urine from bull 3368 was collected on the sixth day after in- 

 oculation — third day of fever — and from bull 3371 at the same 

 stage of the attack. The total amounted to 450 cubic centi- 

 meters, was diluted with water to 1,000 cubic centimeters, and 

 sprinkled on the grass on May 8, 1912, at 4.45 p. m. 



The plot was left twenty-four hours, after which bull 3373 

 was picketed on the ground for fourteen hours. No check was 

 used for infectivity of the urine at the time of voiding. Bull 

 3373 did not become infected ; its susceptibility was demonstrated 

 later. 



The urine of bull 3368 was tested on the seventh day after 

 injection with negative results; the susceptibility of the exposed 

 animal was demonstrated later. 



The mixed urine of bulls 2368 and 3371, if infective at all, 

 did not remain so after twenty-four hours' exposure on grass. 



Experiment 20. — The purpose of this experiment was identical 

 with that of the preceding. 



Urine from bull 3389 was collected on the fifth day after 

 inoculation, second day of febrile temperature, in the amount of 

 500 cubic centimeters, and diluted with water to 1,500 cubic centi- 

 meters. The mixture was sprinkled on the grass at 4.45 p. m. 

 on June 25, 1912. The plot was left unoccupied for thirty-six 

 hours, the weather during this time being cloudy with showers. 



On June 27, at 4.45 a. m., bull 3394 was picketed on the plot 

 and left for twelve hours, after which it was placed in a screened 

 stall. The animal displayed a rise of tem.perature on July 2 

 and diarrhoea with inappetence on July 5. Death occurred on 

 July 9. 



It is concluded that the urine remained infective when spread 

 on grass for thirty-six hours. 



