﻿ix.B, 1 Ward et al.: Transmission of Rvnderpest 69 



Experiment 21. — The purpose of this experiment was similar 

 to that of the ones immediately preceding. The urine was voided 

 by bull 3374. 



Urine to the amount of 150 cubic centimeters was collected 

 on the eighth day after inoculation, second day of febrile tem- 

 perature. This was diluted to 1,000 cubic centimeters, and was 

 sprinkled on the grass at 4.45 p. m. on June 18, 1912. The plot 

 was left unoccupied for thirty-six hours, and no control was used 

 for infectiveness. 



At the expiration of this period, bull 3392 was placed on the 

 grass for twelve hours and did not contract the disease. The 

 susceptibility of this animal was proved later. 



It is concluded that, if the urine was infective on the date 

 collected, it did not remain so under the conditions afforded on 

 the grass. 



INFECTIVENESS OF URINE AND F.ECES SPRINKLED ON GRASS 



Experiment 22. — This experiment differed slightly from the 

 preceding, in that fseces diluted with water were also sprinkled 

 on the grass. The urine and fseces were obtained from bull 3391 

 on June 26, 1912, which was the eighth day after inoculation and 

 third day of febrile temperature. Two hundred cubic centi- 

 meters were diluted with water to 1,000 cubic centimeters and 

 sprinkled upon the grass at 5 p. m. The plot was left unoccupied 

 for forty-eight hours, and no control was used to test the in- 

 fectiveness of the excreta at the time when voided. 



After forty-eight hours bull 3396 was picketed on the spot for 

 twelve hours, but did not contract the disease. Susceptibility 

 of this animal was proved subsequently. 



On the twelfth day after inoculation, 500 cubic centimeters 

 of faeces were diluted with water to 1,000 cubic centimeters and 

 sprinkled on the grass. After twenty-four hours, during which 

 time the weather was cloudy, bull 3400 was picketed on the spot ; 

 it failed to contract the disease. Very likely the excreta when 

 voided were noninfective, due to the late stage of the disease. 



It is concluded that, if the faeces and urine were infective at 

 the time voided, the infection did not survive on the grass for 

 twenty-four hours under the conditions existing. 



INFECTIVENESS OF F^CES SPRINKLED ON GRASS 



Experiment 23. — This experiment was designed to test the 

 duration of vitality of rinderpest virus in faeces diluted in water 

 and spread on grass. Material was collected from bull 3448. 



