﻿70 The Philippine Journal of Science mt 



On June 26, at 5 p. m., which was the sixth day after inocula- 

 tion and fourth day of febrile temperature, 550 cubic centimeters 

 of faeces were collected and diluted to 1,500 cubic centimeters. 

 This mixture was sprinkled on the grass which was left for 

 twenty -four hours, during which time the weather was rainy. 

 At the expiration of this period, bull 3392 was picketed on the 

 spot for twelve hours, after which it was removed and placed 

 in a screened shed. The first rise of temperature occurred on 

 July 2, while diarrhoea and inappetence occurred on July 5. 

 Death from rinderpest occurred on July 9. 



On July 30, 1912, which was the tenth day after injection and 

 eighth day after rise of temperature — and two days before 

 death — 600 cubic centimeters of fasces were collected and diluted 

 to 1,000 cubic centimeters in water. This was sprinkled on the 

 grass at 4.15 p. m., after which the grass plot was left for 

 twenty-four hours, during which time the sky was cloudy at 

 times. Following this interval, bull 3399 was placed on the grass 

 for twelve hours, after which it was isolated in a screened stall. 

 No disease developed, and afterwai'd the animal was proved to 

 be susceptible. 



It is concluded that the rinderpest virus in the faeces remained 

 infective on the grass for twenty-four hours at an early stage 

 of the disease, but not at a later stage. 



INFECTIVENESS OF FAECES AND URINE AT VARIOUS STAGES 



OF AN ATTACK 



Experiment 2^. — This experiment was designed to determined 

 on what days faeces and urine from carabaos suffering from 

 rinderpest are infective to cattle. For this purpose faeces and 

 urine were obtained from carabao 3177 during an attack of 

 rinderpest, on the fifth day after inoculation and first day of 

 febrile temperature. The details of the work appear in the notes 

 below: 



AugTist 21, 1911. Two hundred cubic centimeters of urine from 3177 

 were given as a drench to 3133, which was immediately afterward isolated 

 in a screened stall. 



August 22. Two hundred cubic centimeters of faeces from 3177 and 

 water in equal parts were given as a drench to 3117, which was imme- 

 diately isolated. 



August 23. No faeces nor urine were collected on this date. 



August 24. No faeces nor urine were collected on this date. 



August 25. Two hundred cubic centimeters of urine from 3177 were 

 given to 3157 as a drench, after which it was isolated. 



August 26. Two hundred cubic centimeters of urine from 3177 were 

 given to 3121 as a drench, after which it was placed in isolation. 



