﻿IX. B. 1 



Ward et <il.: Trans-mission of Rinderpest 



71 



August 27. Two hundred cubic centimeters of faeces from 3177 and 

 water in equal parts were given to 3125 as a drench, after which it was 

 isolated. 



August 28. Two hundred cubic centimeters of urine from 8177 were 

 given to 3192, which was then isolated. 



It was decided, on account of the expense, not to attempt to 

 infect more animals from 3177, but to use the results obtained 

 in this experiment as a guide for future work. 



Table X. 



—Tests 



of infectivity of fseces cmd urine. 



Date. 



Animal 

 No. 



Material. 



Result. 



Susceptibility 

 test. 



Aug. 21.... 

 Aug. 22.... 

 Aug. 23 



3,133 

 3,117 



Urine . 



Negative 



do 



Susceptible. 

 Do. 



Susceptible. 







Aug. 24 









Aug. 25--.. 

 Aug. 26..- 

 Aug. 27.... 

 Aug. 28.... 



3,157 

 3.121 

 3.125 

 3,192 



Urine 



do 



Faeces 



Urine 



Positive 



do 



Negative .-. 

 Positive 



The relation existing between the stage of the disease and the 

 infectivity of the faeces and urine is shown in graphic form on 

 the temperature chart of 3177, Plus or minus signs indicate 

 whether or not faeces and urine collected on the respective days 

 induced the disease in other animals. 



The disease apparently had not progressed enough on August 

 21 and 22 to render infective the urine and fseces tested on these 

 dates, respectively. Fseces were noninfective on August 27, 

 while urine of the next day was infective. 



DURATION OF INFECTIVENESS OF VIRUS IN WATER 



Experiment 25. — This experiment was designed to furnish 

 data as to the duration of viability of rinderpest virus in faeces 

 and urine when mixed with water. 



Faeces and urine were collected on September 23, 1911, from 

 bull 3207, on the ninth day after inoculation and the fifth day 

 succeeding the initial rise of temperature. The animal had ex- 

 perienced an acute attack of rinderpest, and died the same day. 

 Some of the faeces were gathered at autopsy. The same material 

 was also collected from bull 3194 on the day of death, which was 

 the eighth day since inoculation with virulent blood and the sixth 

 day following the initial rise of temperature. 



A mixture of fseces and urine from 3207 and 3194 was placed 

 in a keg in equal parts with water, and the mixture was placed 

 in the shade. 



