﻿IX, B, 1 Ward et al.: Transmission of Rinderpest 77 



lation, four days after rise of temperature, when diluted with 

 water and sprinkled on grass, proved to be infective twenty-four 

 hours later. Faeces from the same animal ten days after in- 

 jection, when tested in the same manner, gave negative results. 



Experiment 2U. — Faeces were collected from an animal during 

 an attack of rinderpest at seven and twelve days after inocu- 

 lation and administered to susceptible animals. The first sam- 

 ple only yielded positive results. Urine collected at six days 

 gave negative results, but that collected at ten, eleven, and thir- 

 teen days produced the disease. 



Experiment 25. — Faeces and urine were collected from an 

 animal during an attack of rinderpest nine days after inocula- 

 tion, five days after rise of temperature. The same material 

 was collected from a case eight days after inoculation, six days 

 after rise of temperature. All was diluted with equal parts of 

 water in a keg in the shade. Rinderpest was produced in ani- 

 mals to which this mixture was administered, immediately, and 

 after thirty-six hours. Tests made at sixty, eighty-four, and 

 one hundred eight hours yielded negative results. 



Experiment 26. — Forty-eight animals that had experienced 

 attacks of rinderpest within three months previously were mixed 

 with 3 susceptible animals for twenty-five days without evi- 

 dence being produced that they were capable of transmitting the 

 disease. 



CONCLUSIONS 



1. Rinderpest virus was not shown to have survived beyond 

 twenty-four hours in corrals bare of vegetation but containing 

 water. The conditions under which tests were made included all 

 seasons of the year with accompanying variation in sunlight, 

 rain, and condition of the soil. The amount of shade varied 

 widely. 



2. Animals became infected in such corrals within half an 

 hour, twelve hours, and seventeen and one-half, respectively, 

 after removal of the sick. 



3. Animals infected with rinderpest were shown to be capable 

 of transmitting the disease to susceptible animals by close con- 

 tact only during the febrile period of the disease, and most cer- 

 tainly during the period in which the temperature was declining. 

 The disease was not contracted by susceptible animals when ex- 

 posed to sick animals during the convalescent stage when the 

 temperature was nearly normal. 



4. Blood of animals infected with rinderpest was shown in 

 two cases to be infected during the height of the febrile period. 



