﻿SIMULTANEOUS METHOD OF INOCULATING CATTLE AND 



CARABAOS WITH SERUM FROM ANIMALS THAT 



HAVE BEEN RECENTLY IMMUNIZED 



By Archibald R. Ward and Frederick Willan Wood 

 (From the Veterinary Division, Bureau of Agriculture, Manila, P. I.) 



Three plates 



The earlier literature concerning antirinderpest serum con- 

 tains frequent references to the idea of increasing the protective 

 power of the serum by successive injections of virulent blood 

 into the animal producing the serum. The idea is emphasized 

 that the serum of such hyperimmunized animals is preferable to 

 the serum from animals that have merely been immunized nat- 

 urally or artificially. 



Braddon(i) states that in South Africa the method of injec- 

 ting uninfected animals with defibrinated blood of animals which 

 had recently recovered from pest, as a preventive method, was 

 abandoned in favor of Turner's method of using serum from 

 animals that had received successive and increasing doses of 

 virus. 



Turner (2) states that in the Transvaal and Natal an immune 

 animal was injected with 100 cubic centimeters of virulent blood, 

 and after all reaction had ceased it was bled. This blood, when 

 defibrinated, was injected into a susceptible animal which was 

 smeared on the muzzle with virulent material and placed with 

 animals suffering with rinderpest. This method of simultaneous 

 inoculation, using blood prepared at the time, seems to have been 

 abandoned in favor of one employing a serum requiring more 

 elaborate preparation. 



Since the use of serum from hyperimmunized animals came 

 into general use in simultaneous inoculation, Gibson (3) is the 

 first writer known to us, who has questioned the necessity of 

 hyperimmunizing serum-producing animals. 



Shealy(4) makes a similar observation to the effect that the 

 results obtained with the serum prepared from animals after 

 recovery from an attack were found to be just as good as when 

 the animal was not bled until it had been hyperimmunized. 



Subsequent to the completion of the field work described 



125 



