﻿IX, B, 1 



Ward and Wood: Inoculating Cattle and Carabaos 129 



Table I. — Record of immunization at Laoag of carabaos belonging to the 



Calamba Sugar Estate. 



Lot No. 



1 



Number 

 injected. 



Date of 

 injection. 



Serum 

 per head. 



Febrile reac- 

 tions. 



Number 

 released. 



Loss after 

 injection. 



No. 



Per 

 cent. 



No. 



Per 

 cent. 



1 



2.. -.- - 



3 - 



8 

 70 

 63 



July 28 

 Aug. 11 

 Aug. 23 

 Aug. 28 

 Sept. 8 

 Sept. 12 

 Sept. 15 

 Sept. 24 

 Sept. 26 

 Oct. 3 

 Oct. 9 



c. c. 

 200 

 300 

 300 



4 

 28 

 48 



50.0 

 40.0 

 76.0 

 66.0 

 56.0 

 86.0 

 20.0 

 15.0 

 19.0 

 43.0 

 66.0 



6 

 70 

 60 

 65 

 27 

 15 

 10 



2 

 

 3 

 





 n 



26.0 

 



4.7 

 

 

 

 

 

 

 

 L5 



4 



6 



6 



7. 



65 

 27 

 15 

 10 

 26 

 31 

 51 

 63 



350 43 

 360 1 15 

 350 1 13 

 350 I 2 

 350 1 4 



8 



26 



9 



350 

 350 

 350 



6 

 22 



42 



31 

 51 

 62 





 



1 



10 



11 



Total - 



429 





227 



52.9 



423 



6 



1.4 















It will be observed that all of the deaths, except one, occurred 

 in the first three lots. In the case of those animals only, serum 

 made in the laboratory from hyperimmunized animals was used. 

 Increase of the dose of serum from 200 cubic centimeters to 300 

 cubic centimeters practically stopped losses. 



It is believed on the basis of the previous work (6) and that of 

 Holmes (5) that an animal may be immunized by simultaneous 

 inoculation without showing either fever or symptoms. There- 

 fore, it is thought possible that more susceptible animals may 

 have been immunized than shown in the table. 



Table II gives data concerning the number of animals in each 

 lot which attained maximum temperatures of a degree corre- 

 sponding to four classes chosen arbitrarily. 



Table II. — Febrile reactions among carabaos at Laoag. 



( 



Maximum. 









Lot No 



.- 









Totals for each 



1 



2 



3 



4 



5 



6 



7 



8 



9 



10 



11 



temperature class. 



40° or over 



4 

 

 

 4 



15 

 12 



1 

 42 



46 

 1 

 2 



15 



33 

 8 

 2 



22 



11 

 3 



1 

 12 



10 

 2 



1 

 2 



1 

 1 

 



8 



2 

 1 



1 

 22 



2 

 2 

 2 



25 



15 

 6 

 1 



29 



20 



14 



8 



21 



Numi>er. 



158 



50 



19 



202 



Per cent. 



"69.6 



^22.0 



a8.3 



1.47.1 



39°.5to39°.9 . .. 



39°to39°.4._- 



Under 39° 



Total number of ani- 

 mals 





























8 



70 



63 



65 



1 



27 IS 



10 



26 



V 



fil 



63 



429 















» Per cent relation to 227, the total number of reactors. 

 >> Per cent relation to 429, the total number injected. 



It will be noted that 46.3 per cent of all failed to reach 39° C. 

 and are not reckoned as having shown a febrile reaction in 

 accordance with the standard described above. Comparatively 

 few attained a temperature between 39° and 39°. 5. 



124293 9 



