﻿254 



The Philippine Journal of Science 



1914 



Equal portions of it and a suspension of typhoid organisms were 

 placed on a cover glass by means of platinum loops of equal size 

 and thoroughly mixed. This constituted the hanging drop used 

 in connection with a depression slide. All glassware was clean 

 but not sterile. In diluting the serum, a separate portion of 

 normal saline solution was used for each specimen. The sus- 

 pension was prepared from an eighteen to twenty-four hour old 

 acid agar slant subculture with normal saline. The hanging 

 drop was carefully ringed with vaseline. Microscopic obser- 

 vation of the hanging drop was made every few minutes. The 

 1 to 80 dilution was prepared by taking equal portions of a 

 1 to 40 dilution of serum and bacterial suspension. 



Noteworthy agglutination and loss of motility were present in 

 49, or 16.1 per cent, of the cases as indicated in Table I. This 

 was of such a degree as to be considered definitely positive in 

 12, or 4 per cent, of the series of cases. The positive cases are 

 numbers 1, 2, 9, 13, 20, 22, 23, 25, 26, 29, 40, and 49 in the table. 



Table I. — Reactions in JtiS of the SOO cases examined. 



[Clumping and loss of motility absent (A), complete (C), and partial (P).] 



No. 



Ori^nal reactions. 



Check reactions. 



Dilution 1 to 40. 



Dilution 1 to 80. 



dilution 1 to 40. 



Minutes. 



Result. 



Minutes. 



Result. 



Minutes. 



1 

 Result. 



1 



40 

 30 

 55 

 60 

 60 

 60 

 60 

 60 

 30 

 60 

 60 

 50 

 20 

 50 

 60 

 60 

 60 

 60 

 60 



45 



60 

 35 



50 



C 

 C 

 C 

 P 

 P 

 P 

 P 

 P 

 C 

 P 

 P 

 C 

 C 

 C 

 P 

 P 

 P 

 P 

 P 



c 

 p 







c 











2 











3 











4 



90 

 90 

 90 

 90 

 90 

 60 

 90 

 90 

 90 

 25 

 90 

 90 

 90 

 90 

 90 

 90 



60 



90 

 90 



60 



P 

 P 

 P 

 P 

 P 

 C 

 A 

 P 

 A 

 

 A 

 A 

 A 

 A 

 P 

 P 



C 



P 

 C 



c 







5 



90 



P 



6 . 



7 



90 

 60 



P 

 P 



8 



9 



10 



90 

 90 

 90 

 60 

 90 

 60 

 90 

 90 

 60 

 90 

 (60 

 l90 

 60 

 25 

 45 



A 

 P 

 P 

 P 

 P 

 P 

 P 

 P 

 P 

 P 

 P 

 C 

 P 



c 

 c 



11 



12 



13 - 



14 



16 



16 



17 



18 



19 



20 



21 



22 



23 ._ 



