﻿260 The Philippine Journal of Science 1914 



4. The substance so obtained is very much more active than that obtained 

 in virulent Blood, so much so that it cannot be given subcutaneously with 

 safety on account of the extreme inflammatory condition it sets up. 



5. To use it as an hyper-immunizing agent, doses are better given intra- 

 venously and gradually increased so that the "salting" is spread over a 

 period of some 2 months or more. 



6. The immune serum so obtained is powerful, but 15-20 per cent, weaker 

 than that made by massive injections of virulent blood. 



7. The method is an eminently practical one and shows a great financial 

 saving. 



Experiments were performed by me with the view of determin- 

 ing whether or not it is possible to obtain the toxin of rinderpest 

 by cultural methods, as claimed by Baldrey. 



Both cattle and carabaos were used. A few were immune 

 to rinderpest, but the majority were susceptible. 



Experiment 1. — This experiment was performed to deter- 

 mine the action of Martin's broth alone upon an animal. Since 

 this medium is highly peptonized, it was thought that possibly 

 it might have some effect upon an animal when given in large 

 quantities. 



The animal used was Batanes bull 3140, which had previously 

 been immunized to rinderpest and had recovered thirty-eight days 

 prior to this experiment. On October 4, 1911, this animal was 

 injected with 700 cubic centimeters of Martin's broth, neutral 

 in reaction to litmus paper. One hundred cubic centimeters 

 were administered intravenously, and 600 cubic centimeters, 

 subcutaneously. 



This animal suffered no apparent ill effects from the injection 

 of the culture medium. It ate heartily, and was thrifty in ap- 

 pearance throughout the experiment. There occurred a slight 

 rise in temperature during the first thirty-six hours following 

 the injection, which may have been due to the peptone content 

 of the broth. 



The result obtained in this experiment would lead one to infer 

 that the broth used in the following experiments, by itself, has 

 practically no effect upon the animals. 



Experiment 2. — This experiment was designed to duplicate 

 the first experiment described in Baldrey's paper, the only differ- 

 ence being the use of Martin's broth instead of ordinary broth. 



The animal used was Batanes bull 3146, which had previously 

 been immunized to rinderpest and had recovered forty days prior 

 to this experiment. On October 7, 1911, this animal received by 

 subcutaneous injection 500 cubic centimeters of Martin's broth, 

 which had been inoculated with 25 cubic centimeters of virulent 

 blood from bull 3217 and kept at 37° C. for twenty-four hours. 



