﻿IX, B, 3 Boynton: Cultivation of Rinderpest Virus 261 



This animal showed no ill effects from the injection, and its 

 temperature was normal throughout the experiment. 



The results obtained do not coincide with those obtained by 

 Baldrey. 



The following experiments were performed with animals 

 which had not previously been immunized to rinderpest. The 

 object was to determine if possible whether the toxin of rinder- 

 pest, if present in the inoculated media, would have any effect 

 upon susceptible cattle and also to ascertain if the virus re- 

 mained alive after being incubated for twenty-four hours at 

 37° C. 



Experiment 3. — The animal used was Batanes bull 3222, 

 susceptible to rinderpest. On October 12, 1911, at 2.30 p. m., 

 this animal received by subcutaneous injection 1,000 cubic centi- 

 meters of Martin's broth which had been handled as follows: 

 Nine hundred fifty cubic centimeters of Martin's broth neutral 

 in reaction to litmus paper were inoculated with 50 cubic centi- 

 meters of defibrinated virulent blood from carabao 3257 and 

 kept in the incubators at 37° C. for twenty-four hours. There 

 was considerable sediment in the broth at the time it was injected, 

 but no microscopical examination was made to ascertain if it 

 was free from visible microorganisms. 



On the evening of October 12, this animal ate very little and 

 appeared uneasy. On the morning of October 13, it presented 

 a slightly accelerated respiration, lay down a greater part of 

 the time, and did not eat. During the afternoon of this day, 

 the animal appeared very sick, and toward evening lay in a 

 sprawled out position. It was observed that several quick res- 

 pirations would be taken in succession, after which the animal 

 would hold its breath for some time, as if in great pain. It 

 could not get upon its feet, and was not able even to hold its head 

 up for any length of time. 



On account of the critical condition, the animal was killed at 

 5 p. m., which was twenty-six hours and a half after it had 

 received the injection. Post-mortem examination revealed 

 marked oedema at the points of inoculation, hasmorrhagic in 

 places. The duodenum was slightly congested; otherwise the 

 internal organs presented no appreciable change. 



The temperature had remained stationary at 38°. 8 C. through- 

 out the experiment. 



Sections were made from parts of the oedematous tissue. The 

 tissue spaces were filled with rod-shaped organisms, which in- 

 dicated that the medium evidently had been contaminated with 



