﻿262 The Philippine Journal of Science i9i4 



some organism other than that of rinderpest. This contamina- 

 tion may have had a marked influence upon the symptoms which 

 the animal presented, and it cannot be stated with any certainty 

 that toxin derived from the rinderpest organisms, as stated by 

 Baldrey, was the cause of the condition of the animal at the 

 time it was killed. 



Experiment U. — As soon as bull 3222 was killed, 50 cubic cen- 

 timeters of the heart blood were injected subcutaneously into 

 bull 3213. This animal suffered no ill effects from the injection, 

 but was afterward proved to be susceptible to rinderpest. This 

 shows that whatever agent caused the condition of 3222, it was 

 not present in sufficient quantity in the heart blood to produce 

 any ill effects upon another animal. 



Experiment 5. — The animal used was susceptible Batanes bull 

 3286. On October 24, 1911, at 9 a. m., this animal received by 

 subcutaneous injection, 700 cubic centimeters of culture broth 

 which had been handled as follows: Nine hundred fifty cubic 

 centimeters of Martin's broth of neutral reaction to litmus paper 

 were inoculated with 50 cubic centimeters of defibrinated virulent 

 blood from bull 3223 and kept in the incubator at 37° C. for 

 twenty-four hours. 



On October 27, the animal was noticed to be voiding a large 

 quantity of blood in the urine. On the evening of October 28, 

 it appeared to be very sick, refused food and water, and stood in 

 a hunched up position with staring coat and with the head down. 

 Respiration was rapid and catchy, the animal uttering a low 

 grunt at the beginning of each expiration. There was a marked 

 oedematous swelling along the abdomen and sides of the body. 

 At 11 a. m., the animal lay in a sprawled out position with its 

 head flat on the floor, presented marked symptoms of asphyxia, 

 and frequently struggled. Death occurred at 12.30 p. m. 



Post-mortem examination revealed a slight congestion of the 

 duodenum. Other paiis of the intestinal tract presented no 

 abnormal appearances. The kidneys were enlarged, markedly 

 congested, contained numerous small haemorrhages, and were 

 very friable in texture. The capsule was easily removed. The 

 liver was congested and very friable. The subcutaneous tissue 

 was markedly (Edematous in the vicinity of the points of in- 

 oculation and in the pendent portions of the body. 



This animal had a rise in temperature to 40° C. on the third 

 and to 40°. 6 C. on the fourth day after receiving the injection. 



Sections made from the oedematous tissue revealed numerous 



