﻿IX, B. 3 Boynton: Cultivation of Rinderpest Virus 263 



rod-shaped organisms in the tissue spaces similar to those found 

 in the cedematous tissue of bull 3222. Hence, the symptoms and 

 death may have been due to the products formed by these or- 

 ganisms instead of the toxin formed by the rinderpest virus. 



Experiment 6. — This experiment was conducted similarly to 

 the former ones with the exception that the broth was faintly 

 alkaline in reaction to litmus paper. The animal used was sus- 

 ceptible Batanes bull 3211. This animal was injected subcuta- 

 neously at 2.30 p. m. on October 27, 1911, with 700 cubic 

 centimeters of Martin's broth which had been treated as follows : 

 Nine hundred fifty cubic centimeters of Martin's broth were 

 inoculated with 50 cubic centimeters of defibrinated virulent 

 blood from carabao 3258 and incubated at 37° C. for twenty-four 

 hours. 



The animal showed a rise in temperature on the morning of the 

 fifth day after injection, and the temperature remained above 

 40° C. until the day of death. On the seventh day, the animal 

 developed a diarrhoea, on the eighth day refused food, and death 

 occurred late in the afternoon of the ninth day. Post-mortem 

 examination revealed a marked congestion of the fourth stomach 

 and duodenum. The caecum was congested and hsemorrhagic in 

 places. The gall bladder was distended and congested, perito- 

 nitis was present, and there were erosions in the mouth. From 

 the symptoms and post-mortem findings, one would be justified 

 in concluding that this animal died from a typical attack of 

 rinderpest. 



The results obtained from this experiment would lead one 

 to infer that the toxin had not developed to such an extent 

 as to have any vital effect upon the animal and that the virus 

 of rinderpest had not been killed by the incubation nor its 

 virulence depreciated in any respect. 



Experiment 7. — This experiment was a duplicate of the 

 preceding one. The virulent blood used to inoculate the medium 

 was taken from carabao 3245. 



The animal used in this experiment was susceptible Batanes 

 cow 3294. This animal was injected subcutaneously at 2 p. m., 

 October 31, 1911, with 900 cubic centimeters of the inoculated 

 medium which had been incubated twenty-four hours at 37° C. 



No abnormal symptoms were present until the afternoon 

 of the fifth day after injection, when the animal showed a 

 pronounced rise in temperature. The temperature remained 

 abnormally high until the day of death. On the eighth day, the 

 animal developed a diarrhoea and ate but little. On the ninth 



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