﻿264 The Philippine Journal of Science isu 



day, it presented a profuse diarrhoea and refused food. On the 

 tenth day, bloody mucous casts were passed and the animal 

 lay down most of the time, dying in the evening. 



Post-mortem examination revealed erosion ulcers in the mouth ; 

 marked ulceration and congestion of the fourth stomach and 

 duodenum; and congestion, ulceration, desquamation, and 

 haemorrhagic areas in the caecum, colon, and rectum. Blood- 

 stained mucous casts were present in the lower colon and rectum. 

 Peritonitis and distention of the gall bladder were noted, and 

 the vagina was markedly congested. 



The incubation period, symptoms, and post-mortem findings 

 lead to the conclusion that this animal died of a typical attack 

 of rinderpest. Evidently the virus was not destroyed nor its 

 virulence attenuated to any appreciable extent by the medium 

 or incubation for twenty-four hours. 



The results obtained from this experiment indicate that the 

 toxin, if produced in the culture medium, was not present in 

 sufficient quantity to have any effect upon either the virus or 

 the animal injected. 



Experiment 8. — This experiment was carried on simultaneously 

 with the preceding one, and was similar with the exception 

 that carabao 3262, supposedly susceptible, was used instead of 

 a bull. 



The animal was injected subcutaneously at 2.30 p. m., October 

 31, 1911, with 900 cubic centimeters of Martin's broth, which 

 had been inoculated with 50 cubic centimeters of defibrinated 

 virulent blood from carabao 3245 and incubated for twenty-four 

 hours at 37° C. 



The injection of the incubated broth had no immediate effect 

 upon the animal. It did not contract rinderpest, whereas the 

 animal in experiment 7, receiving medium inoculated with blood 

 from the same animal, contracted the disease. This carabao 

 was later injected with 50 cubic centimeters of virulent blood, 

 but did not contract rinderpest. This leads to the conclusion 

 that this animal was immune to the disease at the time of the 

 experiment. 



From these results, it will be noted that the culture had no 

 visible effect upon this animal. 



Experiment 9. — This experiment varied slightly from the 

 preceding one in that the inoculated medium was incubated 

 forty-eight hours, which gave double the time for the elaboration 

 of toxin. 



The animal used in the experiment was susceptible Timor 



