﻿IX, B. 3 Boynton : Cultivation of Rinderpest Virus 265 



bull 3293, It was injected subcutaneously at 3 p. m., November 

 10, 1911, with 900 cubic centimeters of Martin's broth neutral 

 in reaction to litmus. The medium had been inoculated with 

 50 cubic centimeters of defibrinated virulent blood from carabao 

 3241, and was incubated forty-eight hours at an average tem- 

 perature of 36° C. 



On the morning of the fourth day after injection, the first 

 rise of temperature occurred, which was followed by a tempera- 

 ture of 40° C. in the afternoon. The temperature remained 

 febrile until the day of death. Diarrhoea developed on the tenth 

 day, at which time the animal displayed inappetence. On the 

 eleventh day, the diarrhoea was profuse and continued until death, 

 which occurred on the morning of the thirteenth day after 

 injection. 



Post-mortem examination of this animal revealed erosion 

 ulcers in the mouth; ulceration and congestion of the fourth 

 stomach ; marked congestion and haemorrhages in the duodenum, 

 caecum, colon, and rectum; and peritonitis and emphysema of 

 the lungs. 



In symptoms and lesions, this animal presented all the ap- 

 pearances of having died of rinderpest. Evidently, if any toxin 

 was formed after the forty-eight-hour incubation period in 

 the culture medium, it was so weak that it had no appreciable 

 effect upon the animal injected and did not kill the virus in the 

 medium or weaken its virulence. 



Experiment 10. — This experiment differed from the preceding 

 one in that the medium used was slightly acid in reaction to 

 litmus and susceptible Timor cow 3292 was employed. The cow 

 was injected subcutaneously at 3.15 p. m., November 10, 1911, 

 with 900 cubic centimeters of Martin's broth, which had been 

 inoculated with 50 cubic centimeters of defibrinated virulent 

 blood from carabao 3241 and incubated at approximately 36° C. 

 for forty-eight hours. 



The animal showed no immediate ill effects from the injection, 

 and did not show a rise in temperature nor display any of 

 the symptoms of rinderpest up to the thirteenth day after 

 injection. 



The susceptibility of this animal was not proved, since it was 

 accidentally killed before being tested. 



From the results of this experiment, it is evident that if there 

 was any toxin formed in the medium it was so slight as to 

 have no ill effect upon the animal. It also appears as if the 

 acid medium killed the virus, although there is no experimental 



