﻿266 The Philippine Journal of Science 1914 



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proof that this animal was susceptible. The fact of its suscep- 

 tibility may be taken for granted, since it was one of fifteen 

 Timor animals which were used at the laboratory and the other 

 fourteen contracted rinderpest and died. " The virulence of the 

 blood used for inoculating the medium was checked up as shown 

 in the preceding experiment. 



Experiment 11. — In this experiment, 950 cubic centimeters 

 of a 5 per cent potassium citrate solution were used instead of the 

 Martin's broth. This solution was inoculated with 50 cubic 

 centimeters of defibrinated virulent blood from carabao 3241 

 and incubated at approximately 36° C. for forty-eight hours. 



The animal used was susceptible Timor cow 3296. It was 

 injected subcutaneously at 3.30 p. m., November 10, 1911, with 

 900 cubic centimeters of this culture. 



The animal suffered no immediate ill effects from this injec- 

 tion, and did not contract rinderpest. It was inoculated later 

 with virulent blood, and was proved susceptible to rinderpest. 



It is concluded from these results that either no toxin was 

 formed in the potassium citrate solution or it was formed in such 

 a small quantity that it produced no ill effects upon the animal. 

 Also, the virus was not able to survive in it forty-eight hours 

 under the conditions existing. The virulence of the blood used 

 in this experiment for inoculating the medium was checked up 

 as the results show in experiment 9. 



Experiment 12. — In this experiment, Martin's broth was 

 strongly alkaline in reaction to litmus paper. The animal used 

 was susceptible Timor cow 3297. It was injected subcutaneously 

 at 3.45 p. m., November 10, 1911, with 900 cubic centimeters 

 of Martin's broth, which had been inoculated with 50 cubic 

 centimeters of defibrinated blood from carabao 3241 and 

 incubated at approximately 36° C. for forty-eight hours. 



This animal presented no immediate ill effects from the 

 injection. It developed a temperature on the sixth day after 

 injection, diarrhoea on the tenth day, inappetence on the twelfth 

 day, and died during the following night. 



Post-mortem examinations revealed erosion ulcers in the mouth 

 and a slight congestion of the fourth stomach and duodenum. 

 The caecum and colon were but slightly changed. The rectum 

 was slightly congested and haemorrhagic, and a slight peritonitis 

 was present. The lesions were not so pronounced as those noted 

 in the preceding autopsies. 



From the results obtained from this experiment it is evident 



