﻿THE PHILIPPINE 



Journal of Science 



B. Tropical Medicine 



Vol. IX 



AUGUST, 1914 



No. 4 



THE PIPETTE METHOD IN THE ISOLATION OF SINGLE MICRO- 

 ORGANISMS AND IN THE INOCULATION OF SUBSTANCES 

 INTO LIVING CELLS 



WITH A TECHNIQUE FOR DISSECTION, STAINING, AND OTHER PROCESSES 

 CARRIED OUT UNDER THE HIGHER POWERS OF THE MICROSCOPE '^ 



By Marshall A. Barber 

 (From the Biological Laboratory, Bureau of Science, Manila, P. I.) 



Two plates and 19 text figures 

 CONTENTS 



Introduction. 



Isolation of Microorganisms. 



General principle. 



Method I. 

 Apparatus. 

 Technique of isolation. 



Method IL 



Method III. 

 Cultivation of the Isolated Or- 

 ganism. 



Cultivation in situ on the cover 

 glass. 



Cultivation in a medium apart from 

 the cover glass. 



Inoculation into animals. 

 Detailed Description of Certain 

 Steps in the Isolation Meth- 

 ods. 



Moisture. 



Danger of contamination. 



Preparation of organisms for iso- 

 lation. 



Nutrient media. 



Illumination. 



Pipettes. 



Gas for the microburner. 

 Special Applications of the Pi- 

 pette Method. 



Isolation of organisms from quan- 

 tities of virater larger than hang- 

 ing drops. 



Fixation and staining. 



Serological tests. 



Experiments on chemiotaxis. 



Dilutions. 



Warm box. 



Dissection. 



The pipette and isolating chamber 

 used as separate units. 



Inoculation into living cells. 



' Received for publication, December 12, 1913. 



128443 



307 



DEC IB tm 



