﻿318 The Philippine Journal of Science 1914 



prepared, sealed at the tips, and kept on the laboratory table 

 ready for use. 



(e) After a suitable point is made, the end of the capillary 

 is turned up at right angles, as shown in a (fig. 6). This is 

 done by holding the part of the capillary just back of the point 

 above the small flame and lifting the point with the tip of the 

 forceps or with a needle. Care must be taken to avoid fusing 

 the glass and thus closing the lumen of the capillary. Not more 

 than 1 centimeter should be turned, as a greater length will be 

 found awkward to manipulate in the moist chamber. If a good 

 point is made, it is not necessary to turn the end at exactly right 

 angles. 



8. After the pipette is finished, attach the rubber tube firmly 

 to the end of the shank and set the pipette in the groove of the 

 holder. Slip the pipette inward, pushing it with the left hand 

 and governing the set screw with the right, until the tip is 

 nearly under the low power of the microscope and is turned in 

 a vertical position. It should be far enough beneath the cover 

 glass to avoid danger of contamination. Set the screw so as 

 to hold the pipette firmly. The exact adjustment of the tip 

 under the center of the low power is attained as follows: Sight 

 along the edge of the cover glass and move the right-and-left 

 adjustment of the holder until the tip is in line with the edge. 

 Then, looking in a direction at right angles to the first, bring 

 the tip to the center of the lens by moving the to-and-from ad- 

 justment of the holder. Or one may make the second adjust- 

 ment by sighting down the side of the tube of the microscope. 

 If the tip is not now in view, it can quickly be found by moving 

 some one of the adjustments of the holder. Focus the low power 

 on the tip, and adjust it to the very center of the field. If desired, 

 the pipette may be adjusted under the low power before the 

 isolation chamber is placed on the stage. The mechanical stage 

 is then moved as far as possible to the right and the chamber 

 put in. One may also remove the eyepiece, turn the objective 

 aside or remove it altogether, and adjust the tip below the empty 

 tube. If it is adjusted to the center of the field, it will be in 

 view on replacing the eyepiece and objective. The first de- 

 scribed method will generally be found preferable. 



9. Lower the tip safely below the level of the hanging drops, 

 focus on the under surface of the cover, and with the mechanical 

 stage bring the edge of one of the drops of sterile broth into 

 the field. Lower the objective until the tip is in view, then 

 slowly raise the objective and tip together until the tip comes 



