﻿360 The Philippine Journal of Science 



Fig. 5. Method of making the capillary pipette, h, microburner. 



6. a, completed pipette, b, c, and d, various sorts of points. 



7. A dissecting microscope used as a pipette holder. 



8. A cover glass with droplets arranged for an extended series of 



isolations, c, a droplet shown on a larger scale with a small 

 bacterium-containing droplet beside it. 



9. Two-movement pipette holder, pipette and isolation chamber ar- 



ranged to illustrate method of transferring single isolated bacteria 

 by means of a perforated mica plate, ms, mica plate; cv, small 

 cover glass over perforation; x, large cover glass; p, pipette; g, 

 up-and-down adjustment of holder moved by screw /; r, to-and-fro 

 movement regulated by screw s. 



10. A capillary tube in position for taking up isolated organisms. 



11. A pipette constructed for use above a barrier of blotting paper. 



12. Special isolating chamber in longitudinal section. The chamber 



b is closed at the side by the trap t and partition p. 



13. A special pipette in position for isolating organisms from the 



bottom of a Petri dish, p and pa, metal plates screwed to the 

 stage for the attachment of the pipette holder. The complete 

 pipette holder is used, but only a portion of it is shown in the 

 figure. 



14. Special pipette for measuring small quantiti-^s of liquid. 



15. Microscope with two pipette holders, each containing a pipette 



attached to the stage by means of metal plates. Seen from the 

 back. A, a three-movement, and B, a two-movement holder. 

 tf, adjustment, governed by screw s", for moving the pipette to 

 and from the observer. The other letters correspond to those 

 shown in figs. 1 and 2. 



16. Apparatus for attracting certain motile bacteria into pipettes. 



17. Pipette for injecting substances into living cells. Completed. 



18. The inoculation pipette in position with the apparatus for regulat- 



ing the temperature of the loop. R, ratchet and pinion of a 

 simple microscope; S, sleeve of metal fastened to the lens holder 

 which is jointed to the microscope at J ; T, hollow metal tube 

 inserted in the sleeve and bearing at its base the cup C. 



19. Various types of tips used in cell injection. 



