﻿IX, B, 5 Walker and Barber: Malaria in the Philippines 



429 



in our report. This will indicate better the reliability of our 

 work, and will be helpful to others who may undertake similar 

 investigations. 



Of these 184 experiments, 79 were with Anopheles febri- 

 fer, 117 with Anopheles rossii, 59 with Anopheles barbirostris, 

 5 with Anopheles sinensis, and 19 with Anopheles maculatus. 

 Each cage contained a variable number of mosquitoes, and many 

 of the cages contained two or more species, just as they had 

 emerged from the larvae collected in one locality. 



NUMBER OF MOSQUITOES DISSECTED 



In the 184 experiments, 1,287 lived to be dissected for the 

 presence of oocysts in their mid-guts; of these, the salivary 

 glands were also dissected in 316 for the presence of sporozoites 

 although as shown in Table V about 50 per cent of the mos- 

 quitoes died during the course of the experiments. These totals 

 are distributed among the several species as follows: 



Table IX. — Distribution of the dissected mosquitoes among the several 



species. 



Species. 



dissected., if 1-^ 



Anopheles febrifer 



Anopheles rossii _ . 



Anopheles barbirostris 



Anopheles sinensis 



Anopheles maculatus.. 



Total 



1.287 



373 



93 



642 



129 



205 



70 



18 







49 



24 



316 



DEVELOPMENT OF OVA IN THE EXPERIMENTAL MOSQUITOES 



Of the 1,287 female anopheline mosquitoes dissected, the ova 

 were developed in 73, not developed in 1,139, and not recorded 

 in 75. As in the case of the mosquitoes in cages 95 to 227a 

 the females were separated from the males shortly after emerg- 

 ing, it is fairer to exclude these from consideration, as the 

 opportunity for copulation was greatly reduced. Of the 630 

 females dissected from these cages only 26 showed development 

 of the ova. In the remaining cages, 1 to 94, the males remained 

 with the females up to the time of dissection. Six hundred 

 fifty-seven females were dissected from these cages, of which 

 the ova were developed in 47 and not developed in 492. No 

 record was taken of the remainder. The distribution of the 

 females having ova developed among the several species is shown 

 in Table X. 



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