﻿434 The Philippine Journal of Science 1914 



subtertian parasite up to the infection of the salivary glands 

 of the mosquito appeared to be slightly longer — from thirteen 

 to fifteen days. No infected salivary glands were found among 

 the mosquitoes dissected that had fed on the blood of patients 

 infected with tertian or quartan malaria. For comparison with 

 these results it is interesting to note that Darling (1910) found 

 sporozoites of the tertian parasite after eleven and one-half 

 days and of the subtertian parasite after eleven days in the 

 salivary glands of Anopheles (Celia) albimamts in the Panama 

 Canal Zone. 



It is noteworthy that, while 18.01 per cent of 111 Anopheles 

 fehrifer dissected between the tenth and eighteenth days after 

 the infective meal showed sporozoites in the salivary glands, 

 none of the 125 Anopheles rossii dissected during the same pe- 

 riods after the infective meal showed sporozoites in the salivary 

 glands. However, oocysts containing sporozoites were found 

 repeatedly in infected Anopheles rossii. 



IV. SUMMARY AND CONCLUSIONS 



Number of experiments, 184. Number of mosquitoes infected, 205. 



With subtertian malaria, 134. Mid-guts, 189. 



With tertian malaria, 26. Salivary glands, 28. 



With quartan malaria, 24. Anopheles febrifer, 132. 



With Anopheles febrifer, 79. Anopheles rossii, 63. 



With Anopheles rossii, 117. Anopheles barbirostris, 7. 



With Anopheles barbirostris, 59. Anopheles sinensis, 0. 



With Anopheles sinensis, 5. Anopheles maculatus, 3. 



With Anopheles maculatus, 19. Percentage of total mosquitoes in- 



Number of malaria patients used, 17. fected, 15.92. 



Subtertian, 8. Anopheles febrifer, 35.38. 



Tertian, 7. Anopheles rossii, 9.81. 



Quartan, 2. Anopheles barbirostris, 3.41. 



Gamete counts (percentage of leuco- Anopheles sinensis, 0. 



cytes), 0.6 to 87.0 Anopheles maculatus, 6.12. 



Number of mosquitoes dissected. Percentage of mosquitoes infected in 



1,287. the strictly comparative experi- 



Mid-guts, 1,287. ments, 32.54. 



Salivary glands, 316. Anopheles febrifer, 66.66. 



Anopheles febrifer, 373. Anopheles rossii, 18.71. 



Anopheles rossii, 642. Anopheles barbirostris, 6.00. 



Anopheles barbirostris, 205. Anopheles sinensis, 0. 



Anopheles sinensis, 18. Anopheles maculatus, 66.66. 



Anopheles maculatus, 49. 



The results of these experiments show that Anopheles febri- 

 fer is probably, among the anopheline mosquito in the Philip- 

 pine Islands, the most susceptible to infection with the parasites 

 of subtertian malaria. While the number of experiments with 



