﻿THE PHILIPPINE 



Journal of Scienc 



B. Tropical Medicine 



Vol. IX NOVEMBER, 1914 No. 6 



THE GERMICIDAL POWER OF GLYCERIN ON VARIOUS MICRO- 

 ORGANISMS UNDER VARIOUS CONDITIONS 



By £. H. RUEDIGER 



{From, the Biological Laboratory, Bureau of Science, Manila, P. I.) 



Although glycerin is extensively used, especially as a preser- 

 vative in vaccine virus, very little has been published regarding 

 its germicidal properties. Rosenau * seems to be the only one 

 who has made any extensive study of these properties. The use 

 of glycerin as a germ-destroying agent in the preparation of bac- 

 terial vaccine introduced by Row - has aroused new interest in 

 the subject. The tests reported here were made in order to 

 obtain accurate information as to the extent that glycerin may 

 be relied upon to sterilize bacterial vaccines. 



THE GERMICroAL POWER OF GLYCERIN IN PHYSIOLOGIC SALT 

 SOLUTION AT ROOM TEMPERATURE 



TEST 1 



Eight sets of test tubes were prepared, and the sets were num- 

 bered 1, 2, 3, 4, 5, 6, 7, and 8. Each set contained four tubes, 

 a, b, c, and d. Into tube a were put 2 cubic centimeters of salt 

 solution (9 grams per liter) ; into tube h were put 2 cubic centi- 

 meters of 12.5 per cent solution of chemically pure glycerin ; into 

 tube c were put 2 cubic centimeters of 25 per cent solution of 

 glycerin; and tube d received 2 cubic centimeters of 50 per cent 

 solution of glycerin. The tubes with their contents were ster- 

 ilized in the autoclave. After sterilization the 8 sets of tubes 

 were inoculated with the typhoid bacillus, Staphylococcus albus, 



^Bull. Hyg. Lab. U. S. Pbl. Hlth. & Mar.-Hoap. Serv., Wash. (1913), 

 No. 16. 



Woum. Trop. Med. (1913), 16, 293. 



129562 465 



