﻿466 



The Philippine Journal of Science 



1914 



Staphylococcus aureus, the bacillus of anthrax, the bacillus of 

 plague, the spirillum of cholera, the bacillus of diphtheria, and 

 with the bacillus of glanders, respectively. Each tube received 

 about one billion organisms. 



For a period of fifteen days a 2-millimeter loopful of the bac- 

 terial suspension from each tube was planted on slanted nutrient 

 agar, and after from three to five days' incubation the results 

 were recorded (Tables I to VIII, inclusive). The plus sign (+) 

 in these tables means that growth of the organisms was obtained 

 on the agar, while the zero sign (0) stands for no growth on the 

 agar. 



This work was done in March, April, and May, 1914, during 

 which time the temperature varied from about 28° C. to 35° C. 

 No tests were made at incubator temperature, 37° C. 



Table I. — The action of glycerin in salt solution at room temperature on 



the typhoid bacillus. 



Glycerin. 



Days. 



1 



2 



8 



4 



6 



6 



7 



8 



9 



10 



11 



12 



18 



14 



16 



Oper cent 



12.6 per cent 



25 per cent 



50 per cent 



+ 

 + 

 + 



+ 

 + 



+ 

 + 



+ 

 + 

 + 

 + 



+ 

 + 

 + 

 



+ 

 + 

 + 

 



+ 

 + 

 + 

 



4 

 + 

 + 

 



+ 

 + 

 + 

 



+ 

 + 

 

 



+ 



+ 





 



+ 



+ 





 



+ 

 + 

 

 



+ 

 + 

 

 



+ 

 

 

 



+ 

 

 

 



Table II. — The action of glycenn in salt solution at room temperature on 



Staphylococcus albus. 



Glycerin. 



Days. 



1 



2 



8 



4 



5 



6 



7 



8 



9 



10 



11 



12 



13 



14 



16 



Oper cent 



12.6 per cent 



26 per cent 



60 per cent 



+ 



+ 

 + 



+ 

 + 







4- 

 + 

 + 

 



+ 

 + 

 

 



+ 

 + 

 

 



+ 

 

 

 



+ 

 

 

 



+ 

 

 

 



+ 

 

 

 



4- 

 

 

 



+ 





 

 



+ 

 

 

 



+ 

 

 

 



+ 

 

 

 



+ 

 

 

 



Table III. — The action of glycerin in salt solution at room temperature on 



Staphylococcus aureus. 

















Days 

















Glycerin. 































1 



2 



8 



4 



6 



6 



7 



8 



9 



10 



11 



12 



13 



14 



16 



Oper cent 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



12. 6 per cent 



+ 



+ 



+ 



+ 



-f 



+ 



+ 



+ 



+ 



+ 



+ 



+ 















26 per cent 



+ 



+ 



+ 



















































60 per cent 



+ 



























































