﻿468 



The Philippine Journal of Science 



1914 



Table VIII. — The action of glycerin in salt solution at room temperature on 



the bacillus of glanders. 



Glycerin. 















Days. 

















































1 



3 



3 



4 



5 



6 



7 



8 



9 



10 



11 



12 



13 



14 



16 



per cent 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



A. 



+ 



+ 



+ 



J- 



! 12.5 per cent 



+ 



+ 



+ 



+ 



+ 



+ 



H- 



4- 



+ 



+ 



+ 



+ 



+ 



+ 



"T 



25 per cent. 



+ 



+ 



+ 



















































j 50 per cent 







' 























' 















° 















It will be seen from the results recorded in Tables I to VIII, 

 inclusive, that the germicidal power of glycerin varies with dif- 

 ferent microorganisms. In 50 per cent of glycerin in physiologic 

 salt solution the typhoid bacillus lived three days ; Staphylococcus 

 albus and aur-eus lived one day ; the plague bacillus, the spirillum 

 of cholera, and the bacillus of glanders were dead before the end 

 of twenty-four hours ; diphtheria bacilli lived two days ; while the 

 bacillus of anthrax was alive at the end of fifteen days. Twenty- 

 five per cent glycerin was sufficiently active on nonspore-forming 

 organisms to receive mention. In 25 per cent glycerin the ty- 

 phoid bacillus lived eight days; Staphylococcus albus, Staphy- 

 lococcus aureus, and the glanders bacillus lived three days; the 

 diphtheria bacillus lived four days ; the spirillum of cholera, two 

 days; while the plague bacillus was dead on the second day. 



TEST 2 



Because of the great importance of the plague bacillus 

 in plague-infected countries, the germicidal power of glycerin 

 in 12.5 per cent, in 25 per cent, and in 50 per cent dilutions in 

 physiologic salt solution, at room temperature, was determined 

 on 4 different strains of plague bacilli. The results are re- 

 corded in Tables IX, X, XI, and XII. 



Table IX. — The action of glycerin in salt solution at room temperature on 



plague ftociiiita No. 2. 



Glycerin. 



Days. 



1 



2 



3 



4 



5 



6 



7 



8 



9 



10 



11 



12 



13 



14 



1 



15 



Opercent 



12.5 percent 



25 per cent 



50 per cent. 



+ 

 + 

 H- 

 



+ 

 + 

 

 



+ 

 + 

 

 



+ 

 

 

 



+ 

 

 

 





 



+ 

 

 

 



+ 

 

 

 



4- 





 

 



+ 





 

 



+ 





 

 



+ 

 

 

 



+ 

 

 

 









 

 





 

 

 



