﻿470 



The Philippine Journal of Science 



1914 



Inoculations made from suspension in 50 per cent glycerin 

 twenty-four hours after the suspensions were made gave neg- 

 ative results. 



TEST 3 



Eight sets of test tubes were prepared as in test 1, and the 

 mixtures were placed in the refrigerator and kept at a tempera- 

 ture of about 15° C. Each day the tubes were removed from 

 the refrigerator to inoculate a tube of agar with one 2-millimeter 

 loopful of bacterial suspension from each tube. The time re- 

 quired for inoculation was short and should not have greatly 

 affected the temperature of the suspension or the action of the 

 glycerin on the bacteria. Tubes of nutrient agar were inoculated 

 daily for a period of fifteen days; the results are recorded in 

 Tables XIII to XX, inclusive. 



Table XIII. 



-The action of glycerin in salt solution at a temperature of 

 15° C. on the typhoid bacillus. 



















Days. 

















Glycerin. 



































1 

 + 



2 



+ 



3 



+ 



4 



+ 



6 



+ 



6 



7 



8 



+ 



10 



11 



12 



IS 



14 



15 



Oper cent 





-f. 



+ 



+ 



-i- 



_l. 



+ 



+ 



+ 



12.5 per cent 



+ 



+ 



-( 



+ 



+ 



+ 



H- 



+ 



+ 



+ 



a. 



■+ 



+ 



+ 



+ 



26 per cent 



+ 



+ 



+ 



+ 



-i- 



+ 



+ 



+ 



+ 



+ 



+ 



"T 



+ 



+ 



+ 



60 per cent 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+■ 



+ 



+ 











Table XIV. — The action of glycerin in salt solution at a temperature of 

 15° C. on Staphylococcus albus. 



Glycerin. 



Oper cent... 

 12.5 per cent 

 25 per cent . . 

 60 per cent . . 















Days. 

















1 



2 



8 



4 



5 



6 



J 



8 



9 



10 



11 



13 



18 



14 



15 



+ 



+ 



+ 



H- 



+ 



+ 



+ 



+ 



.;. 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



o- 



-U 



_1. 



j- 



+ 



-\- 



+ 



+ 



+ 



+ 



J. 



T 



j- 



+ 



+ 



+ 



+ 



+ 



+ 



-i- 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



H- 



H- 



4- 



+ 



+ 



+ 



-1- 



+ 



+ 



+ 



-f 



' 



















Table XV. — The action of glycerin in salt solution at a temperature of 

 15° C. on Staphylococcus aureus. 



Glycerin. 



Days. 



1 



a 



S 



4 



6 6 





8 



9 



10 



11 



12 



13 



14 



15 



Oper cent 



12.5 per cent 



25 per cent 



60 per cent 



+ 

 + 

 + 



H- 



+ 

 + 

 + 



+ 



+ 

 + 



+ 

 + 



+ 



+ + 

 + + 



+ 

 + 

 + 

 -1- 



+ 



+ 

 H- 



+ 



T 



+ 



: 



-1- 



J. 



+ 



+ 

 + 



+ 



+ 



+ 



+ 



-r 

 + 



+ 



+ 

 + 

 + 

 + 



