﻿472 



The Philippine Journal of Science 



1914 



Table XX. — The action of glycerin in salt solution at a temperature of 

 15° C. on the haciUus of glanders. 



Glycerin. 



Days. 1 



1 



3 



3 



4 



5 



6 



7 



8 



9 



10 



u 



12 



18 



14 



16 



Oper cent.- 



12.6 per cent 



26 per cent 



50 per cent 



+ 

 + 



+ 

 + 



+ . 



+ 



+ 



+ 

 + 

 + 



4- 

 + 

 + 

 + 



+ 

 + 

 + 

 + 



+ 

 + 



-V 



+ 

 + 

 + 

 + 



+ 

 + 

 + 



+ 

 + 



+ 

 + 



+ 



+ 







+ 

 + 

 + 

 



+ 

 + 







+ 

 + 







~1" 



» 1 



The results obtained and recorded in Tables XVIII to XX, 

 inclusive, show that at low temperature the germicidal power 

 of glycerin in physiologic salt solution is very slight. All the 

 organisms except the spirillum of cholera lived fifteen days in 

 25 per cent glycerin. In 50 per cent glycerin these organisms 

 lived too long to be of any practical value in the preparation of 

 bacterial vaccine. 



TEST 4 



Eight sets of test tubes were prepared as in test 1, the gly- 

 cerin being diluted with nutrient broth (bouillon) instead of with 

 physiologic salt solution. Each tube of diluted glycerin was in- 

 oculated with about 1,000,000 bacteria. The suspensions were 

 kept at room temperature, and tubes of nutrient agar were in- 

 oculated daily with the suspensions of bacteria in the diluted 

 glycerin. The results are recorded in Tables XXI to XXVIII, 

 inclusive. 



Table XXI. — The action of glycerin in bouillon at room temperature on 



the typhoid bacillus. 



Glycerin. 



Days. 



1 



2 



8 



4 



5 



6 



7 



s 



» 



10 



11 12 



1 



13 



14 



1 

 15 



Oper cent 



12.5 per cent 



25 per cent.- 



50 percent 



H- 

 + 

 + 



+ 



+ 

 + 

 + 



+ 



+ 

 + 

 + 



+ 



+ 



+ 

 



+ 



+ 

 



+ 

 + 

 + 

 



+ 

 







+ 



+ 

 



+ 



+ 







+ 

 + 

 + 

 



+ 

 + 



H- 

 



+ 

 + 

 + 

 



+ 



+ 

 



+ 

 + 







Table XXII. — The action of glycerin in bouillon at room temperature on 



Staphylococcus albus. 



Glycerin. 



per cent 



12.6 per cent 

 25 percent.. 

 60 per cent.. 



1 















Days. 















2 



8 



4 



6 



6 



7 



8 



9 



10 



11 



12 



13 



14 



15 



■f 



+ 



-i- 



+ 



+ 



+ 



+ 



+ 



+ 



H- 



+ 



-L 



+ 



+ 



1 

 + 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



U. 



+ 



+ 



+ 



+ 



































