﻿474 



The Philippine Journal of Science 



1914 



Table XXVII. — The action of glycerin in bouillon at room temperature 



on the bacillus of diphtheria. 



















Days. 

















Glycerin. 

































1 



2 



3 



4 



5 



6 



7 



8 



9 



10 



11 



12 



18 



14 



15 



Oper cent—- 



-L 



+ 



-L. 



+ 



+ 



+ 



_ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



12.5 per cent 



+ 



+ 



-L 



+ 



+ 



+ 



4- 



-f 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



25 per cent. 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



j- 



+ 



"T 



+ 















50 per cent 



+ 



+ 



_1_ 



+ 



-f 



+ 







































Table XXVIII. — The action of glycerin in bouillon at room temperature on 



the bacillus of glanders. 



Glycerin. 



per cent... 

 12.5 per cent 

 25 per cent . . 

 50 per cent.. 



Days. 



10 



11 



18 



13 



14 



16 



From the results recorded in Tables XXI to XXVIII we learn 

 that glycerin mixed with bouillon has but feeble germicidal 

 power. In 12.5 per cent of glycerin, all the organisms lived 

 fifteen days. The typhoid bacillus, Staphylococcus albv^, Sta- 

 phylococcus aureus, and the anthrax bacillus were alive in 25 

 per cent glycerin at the end of fifteen -days. In 50 per cent 

 glycerin the typhoid bacillus live three days, Staphylococcus 

 albus lived four days, Staphylococcus aureus lived five days, the 

 bacillus of anthrax lived fifteen days, the bacillus of plague lived 

 one day, the spirillum of cholera was found to be dead at the 

 end of one day after inoculation, and the bacillus of diphtheria and 

 the bacillus of glanders lived six days and four days, respectively. 



TEST 5 



Eight sets of test tubes were prepared as before. Horse serum 

 was used as a diluent for the glycerin. The tubes were inoculated 

 and were kept at room temperature, and for a period of fifteen 

 days a 2-millimeter loopf ul of bacterial suspension from each tube 

 was transferred to a tube of agar. The results are recorded in 

 Tables XXIX to XXXVI, inclusive. 



