﻿IX, B, 6 Gibson: Physiological Action of the Proteoses 501 



produced the typical fall in blood pressure and inhibition of blood 

 clotting. The amounts necessary to give these results are the 

 same as those usually described (0.3 to 0.5 gram per kilogram 

 of body weight).'' No essential difference was noted between 

 the effects of preparation 1 and the purer preparation 2. 



Proteose preparation 1. — June 24, 1914. Dog, male, weighing 

 4.9 kilograms. Blood samples obtained at 10.30 a. m. clotted in 

 fifteen minutes. Blood pressure at 10.39 a. m. was 130 milli- 

 meters. At 10.40 a. m., the dog received an injection into the 

 saphenous vein of 2 grams (in 20 cubic centimeters of water) , 

 or 0.4 gram per kilogram of body weight of the alcohol-soluble 

 proteoses. Blood pressure fell rapidly at first and then slowly 

 to 30 millimeters of mercury ; at 10.45 a. m. it was 50 millimeters 

 only. Blood samples collected in duplicate at 10.45 a. m. and 

 10.50. a. m. failed to clot. Blood pressure at 11.00 a. m. and 

 11.05 a. m. had risen to 62 and 92 millimeters of mercury. 

 Blood samples obtained at 11.26 a. m. clotted after twenty-five 

 minutes. 



Proteose preparation 2. — June 25, 1914. Dog, female, weigh- 

 ing 8.2 kilograms. Blood samples obtained at 12.03 p. m. clotted 

 in fourteen minutes. Blood pressure at 12.04 p. m. was 148 

 millimeters of mercury. At 12.05 p. m. the dog received an 

 intravenous injection as above of 3.5 grams of the proteoses 

 (0.4 gram per kilogram of body weight) in 40 cubic centimeters 

 of water. Blood pressure fell regularly to 24 millimeters of 

 mercury. At 12.06 p. m. the blood pressure was still 24 milli- 

 meters of mercury. Blood samples obtained at 12.10 p. m. and 

 12.15 p. m. failed to clot. Blood pressures at 12.20 p. m., 12.25 

 p. m., and 12.30 p. m. were 60, 72, and 100 millimeters of mer- 

 cury, respectively. Blood samples collected at 12.36 p. m. still 

 failed to clot. 



CONCLUSIONS 



1. The preparation of semicrystalline alcohol-soluble proteoses 

 from Witte's peptone is described. 



2. When injected intravenously into dogs, these proteoses 

 produce the typical fall in blood pressure and inhibition of blood 

 clotting which have been repeatedly described for other proteose 

 preparations. 



' Schmidt-Miilheim, Arch. f. Physiol. (1880), 33; Fano, ibid. (1881), 277; 

 Chittenden, Mendel, and Henderson, Am. Joum. Physiol. (1899), 2, 149; 

 Underbill, loc. cit., and others. 



