G, F. Barker—Physiological Chemistry. 269 
¢levation of temperature alone will not produce the ferment 
and transform the starch ; a large number of frogs were kept 
for more than six months and no sugar was formed though 
the hepatic cellules were full of starch ; (9) in some cases these 
globules may be absorbed without producing dextrin or sugar ; 
they appear to suffer a change which gives rise to oxalic acid ; 
(10) the diseased livers of mammals, of birds and. of frogs 
the glycogenic transformation is carried on ; (11) during hiber- 
nation, both the ferment and the hepatic starch are present, 
though the latter is diminished in amount; (12) nitrogenous 
tissues treated with cane sugar and sulphuric acid become yel- 
low and then reddish-purple ; since therefore the walls of the 
hepatic cellules and not their contained globules, are colored 
nard’s glycogenic matter according to Schiff, is not a pre-exist- 
ant anatomical element, but a product derived from this by the 
processes employed. 
.) Bernarp followed this paper by a recapitulation of 
the dates of his own discoveries.* First, in 1854, he announced 
development of the tissues of both kingdoms of nature. 
Second, in 1855, he published an experiment fundamental to 
e glycogenic theory ; showing that when the sugar of a 
healthy liver was removed by washing, the saccharine substance 
Was renewed ; thus proving that the sugar is not formed by the 
a er yese 
analogous to that taking place in vegetables. Third, in 1857, 
the isolation of the siicancnt matter was communicated to the 
is paper was antedated and is actually posterior in date to this. 
The glycogenic matter was here called amidon anima ; and 7 
it was shown to be an isomer of vegetable starch, having all 
its physical, chemical, and physiological properties, changing to 
dextrin and suear by acids and ferments, rotating the polarized 
tay, ete, The existence of the ferment both in the liver-tissue 
* ©. R,, xlviii, 884. 
