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G. EF’, Barker—Physiological Chemistry. 395 
frozen portion of the liver, examined 3 hours afterward, yielded 
sugar, The seventh experiment was quantitative ; a dog, pre- 
viously fed on meat, received a full meal of bread and milk, 
and five hours afterward was pithed. A portion of the liver 
was at once sliced off and placed in a freezing mixture. Some 
portal blood collected at the same time afforded a small amount 
of sugar,-derived evidently from the food. Two hours after- 
ward a portion of the frozen liver, and a portion of that which 
had remained warm in the body of the animal, were weighed, 
and the sugar in each was determined volumetrically. The 
frozen piece of the liver contained 0°333 per cent of sugar ; the 
other portion afforded 1:55 per cent ; showing a five fold in- 
crease in 2 hours. But 0°333 per cent is no inconsiderable 
quantity, since in a human liver weighing 50 ounces, it would 
amount to seventy grains of sugar. He concludes as follows : 
| dogs always contains sugar ; 5th, Under favorable conditions, 
Sugar exists in the liver of animals after three days fasting ; 6th, 
this sugar comes partly from the food, partly from the liver, 
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Some days, no sugar was detected in the blood drawn from the 
Tight side of the heart ; (3.) In three dogs, fed on mixed diet, 
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