G. Child on the production of Organisms in closed vessels. 385 
anything which my garden afforded, and in this way pabebly 
my selection of sage and celery may have been a bad one, as 
the aromatic ingredients of these plants may be supposed to in- 
fluence the result of the experiment, especially as in a close 
vessel any volatile oil would be retained. If, therefore, the 
three experiments with these substances be eliminated, there 
remain seven experiments, one with pea-meal, two with hay, 
and four with coarse flour. Of these, five were examined on 
Sept. 19, and in three (viz. the pea-meal and the two hay exper- 
iments) the vessels were found to contain moving organisms. 
In two (those performed with coarse flour) none were found, 
and in the remaining two, examined on Nov. 16, also none were 
ound, . 
In the meantime, when, from several of the above experi- 
ments having produced negative results, I looked upon the 
series as inconclusive, I instituted a fresh series of twelve exper- 
iments in the end of September, as follows. 
The apparatus employed was the same as that used in the 
last series, except that I had some large double bulbs made for 
the present series, In other respects the process was the same 
as before. 
Exp, 1. Sept. 30.—Hay infused 34 hours in water, filtered, and boiled 10 
minutes in a stream of heated air—sealed up when cool. 
Exp. VIII. Oct. 5.—Similar. : 
Exp. IX. Oct. 7.—Flour infused 34 hours, not filtered: boiled 10 min- 
utes in a stream of oxygen, and sealed as before. 
Exp. X. Oct. 7.—Similar: boiled 104 minutes, 
Exp. XI. Oct. 7.—Flour infused 44 hours and filtered: boiled 10 min- 
utes in oxygen. 
Exp. XII.—Similar. 
On Oct. 8 this series of SE PPRIEE SS was divided into two 
sets: [B], Nos, II, IV, VI, VI 
? 
Am. Jour. Sct.—SEconp Serres, Vou. XLI, No. 123,—Mar, 1866. 
49 
