R. H. Chittenden — Papoid-digestion. 303 



From these results, it is manifest that papoid will digest and 

 dissolve cooked beef proteids more readily even than it dissolves 

 coagulated egg-albumin, since the above results were obtained in 

 5y hours digestion at 45° C, while the slightly higher results given 

 for the coagulated albumin were obtained after 1 2 hours digestion 

 at the same temperature. It is further noticeable that the ferment 

 acts most energetically, as in the preceding series of experiments, 

 in the presence of 2-4 per cent, sodium bicarbonate, while a 

 slight addition of acid increases the solvent action a trifle over that 

 of the neutral solution. 



c. Action on Raw Beef Proteids. 



The beef used in this series of experiments was simply hashed, 

 lean beef, washed with water until it was completely free from all 

 soluble matters and nearly or quite white in color. 



Each digestive mixture contained 0*5 gram papoid, 10 grams of 

 the prepared beef, and 25 c. c. of water containing the percentages of 

 acid and alkali indicated. 



The 10 grams of beef contained 2*8508 grams of dry proteid 

 (dried at il0° C.) 



The digestions were kept at 45° C. for Y hours. 



Reaction. 



Weight of 

 undigested residue. 



Raw beef 

 proteids digested. 



Neutral 



0-8988 ; 



gram.. 



68 -4 per cent. 



0'05 per cent. Hydrochloric acid 



0-8910 



a 



68-7 



0-10 " " " 



1-0901 



i i 



61-7 



0-20 



2-0145 



a 



29-3 



2-00 " Bicarb, soda 2 



;ood diges 



tion, bu 



t the residue w 



slimy it could not be filtered. 



4-00 



■Without papoid. 



0'2 per cent. Hydrochloric acid 2*5576 grams 10*3 per cent. 



2*0 " Bicarb, soda 2*7596 '* 3*2 " 



Here, as in the preceding experiments, there is evidence of vigor- 

 ous digestive action especially pronounced in the neutral solution, 

 although still marked in the presence of both dilute acid and alkali. 

 In fact, it is to be presumed, from analogy, that the action of the 

 ferment was even greater in the alkaline solution than in the neutral 

 fluid, although no numerical data were obtained bearing on this 

 point. 



