J. Wyman on living organisms in heated water. 161 
of beef juice were divided into six series of four each, and boiled 
for different periods as cre in the following table; this 
table also gives the day of the x the of the infusoria in 
each of the flasks belonging to a given se 
| Series. nara neg ede 
4 h 30" 4 6 0 
I. ob 45™ 4 4 4 4 
IIL. 1" 00 4 4 4 0 | 
IV. 15" ee SR a 
V. 15 30™ 4 0 0 0 
| VI. Qh .00 0 0 0 0 
Seven of the flasks produced no infusoria, and the number in 
which they did appear becomes less the longer the boiling was 
continued. 
Exp. XTIT.—Of seventeen flasks containing beef-juice eleven 
Were boiled 45 minutes, and 6 were boiled 2 hours. Infusoria 
appeared in all of the first series except one, at the end of the 
second day, and in the remaining one of this series and in all of 
the second on the 3d day. 
1 se It will be seen at a glance that Expts. XII and XIII differ 
In their results, and that of four flasks in Ex. XII boiled 1h. 
and 80 m., three’ produced no infusoria, while in Ex, XIII t 
Weber appeared in all, six of which were boiled two hours. 
e have several times met with similar differences in successive 
properly eg and that there was no error as to the time for 
which the boiling was kept up. 
Exp. XIV.—Twenty ‘pale containing éxtract of beef were 
treated as follows: 
No. of flasks in; Time Day on which infusoria 
each seri*s. boiled. appeared. 
Series I Sflasks. | 1" | 6 6 6 6 6 
: 5 gh gs © 6% 
it aes eis 7; 6 6 OO 8 
Spig 3k 4 5: -* ia oe oC 
: p 
“a ng Vibrio were es in so numbers. 3 
risa ee entnued on the 10th day, as there was no indcaae we of. 
rT chan 
oe XV.— Beer flasks gee a ager solution of : 
of beef”* were arranged in six holes ni 
a cies ae wees wus 
ashe 
