162 J. Wyman on living organisms in heated water. 
No. of fiasks in Time Day on which Infusoria 
each series. boiled. appeared. 
Series I. 5 0° 30™ 1 1 2 2 2 
oeeeas & Ol 1 00 2 2 2 ee | 
+ hk & 1 30 2 y Reis: 2 2 
gee 5 2 20 L-.2-.-2..- 2-4 
Mo, 5 3 30 2 2 3 ey 
« VIL 7 4 00 1 ped ota hom 
An infusorial film formed in all the flasks on the days re 
ted, except in the one marked doubtful in series V, and thos | 
series VI, in which infusoria appeared on the 4th day. Nous of 
these last had a film, but, as in the one marked doubtful in series 
ad an immense number of minute bodies, which formed 4 | 
cloud when the flask was shaken, and were not there when 
experiment was begun. These bodies were spherical, had aa 
Oster wall, and a nucleus which was also a hollow Th 
re of different sizes, and seemed to be undergoing multipl- 
etic by division. Their real nature must be considered doubt- 
ful. The films in the other flasks consisted chiefly of Bacteriums. 
The preceding experiments show that if the boiling of the 
flasks be continued for four hours, as in Exp, XV, the infusoria 
may appear nevertheless—though in other cases it has 
pened, as in Exp. aH, tat ip ceased to be manifested if it was 
continued only for tw 
shing the cake still further, we have not found 
that infusoria appeared in PY instance if the boiling was pro 
longed to five or six hours. Several experiments, in a 
many flasks were used, were fe thio bee the repalt was uniform roaly 
the same, Thus a limit to the development of infusoria in boil 
ae water ae reached. 
aban of ‘the Water's 2° F. If not, then the “invasion of 6 
the contents of the aie by them is easily acco accounted for, since 
experiment pepe that AP ee a very few individuals if present 
tion in a elie Boot ut on the other hand, if Oe ee infu- 
soria perish na the’ P oat then any subsequent bg = 
of them under the circu mstances, mus eee in gome 
t be 
other pc fs than by the common process of reproduction 
‘Sin numbers of infusoria can be proved at all times 19 
be Boating i in the snd Ma we must assume that inl eu inate 
in ever 2 
since certain ——— 
