J. Wyman on living organisms in heated water. 167 
one hand, and the simple exposure of the solution to the air 
without the addition of either, on the other. When an open 
ttle was used it was in most cases covered with a paper cap to 
exclude in a measure the dust from the air; this exclusion was 
of course incomplete, but as all the flasks were equally exposed 
and large numbers were used, any disturbance would be the 
same for all, and might therefore be left out of the account, 
As will be seen further on we have not depended solely on this 
form of the experiment. 
Exp. XVI.—Kighteen bottles of the capacity of 25 c.e. and 
an inch in diameter throughout and containing equal quantities 
of boiled and filtered beef juice were divided into three series of 
sixeach. All of series I were set aside as criteria; to all of. 
series IT were added from a bottle five drops of water containing 
large numbers of living Vibrios taken from a flui 
esh was macerating ; to all of series III were added five drops 
of the same infusorial fluid after it had been boiled five minutes. 
On the 2d day all of series II, to which the unboiled infusoria 
een added, were turbid from the rapid multiplication of 
the animalcules, and an infusorial film was form 
face of the fluid. All of series I and III were unchan ed. 
On the 8d day all of I and II had begun to be turbid, and 
On the 4th were equally so with II. 
: - XVIL—Thirty-three bottles prepared and arranged as 
mm the preceding experiment were divided into three series of 
eleven each, 
. /n the 2d day all of series II were turbid and had an infuso- 
‘al film. The others were unchanged. ; 
On the 8d day eight of series I and seven of series III had 
become turbid and the rest became so on or before the 6th day. 
Which unboiled infusoria were added, were in all cases invaded 
at least one day and in some, two or three days earlier. 
Th each of the receding experiments the bottles were all 
more or less exposed to the air, for the paper covering was only 
» Partial protection. The following modification of them was 
ited, for the purpose of ascertaining if there would be any dif 
ference in the time in which infusoria would appear in portions 
of the same solution enclosed in two series of flasks, to one 
i infuso 
ria had been added, and all of both series sealed at 
of dust from the air was eliminated, excepting in so far as It 
Was present in the flasks at the time they were sealed. This 
